Pathologica · 2000
[Simultaneous detection and typing of human papillomavirus in cervical biopsies using PCR-reverse hybridization].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
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Laconi S, Greco M, Milia G, Pellegrini-Bettoli P, Pani P, Laconi E, Rais M
Аннотация
INTRODUCTION: Genotyping of Human papillomavirus (HPV) is an important step in the clinical evaluation of the oncogenic risk associated with HPV infection of cervical mucosa. The purpose of this work was to develop a fast PCR-reverse-hybridization assay (PCR-RH) for the simultaneous detection and genotyping of anogenital HPVs.
METHODS: HPV DNA from cervical biopsies was amplified by consensus primer-PCR. Digoxigenin-labeled PCR products were hybridized to type-specific probes anchored to the surface of plastic microwells and revealed by an ELISA system.
RESULTS: The method was tested on 115 clinical samples (81 koilocytic atypias, 11 CIN1, 10 CIN2, 12 CIN3 and 1 squamous carcinoma). HPV DNA was found in 56.7% koilocytic atypias, in 90.9% of CIN1 and in 100% of CIN2 and higher-grade lesions. Thus, PCR-RH is sensitive, rapid, easy-to-perform and readily applicable to the routine analysis of a large number of samples.
METHODS: HPV DNA from cervical biopsies was amplified by consensus primer-PCR. Digoxigenin-labeled PCR products were hybridized to type-specific probes anchored to the surface of plastic microwells and revealed by an ELISA system.
RESULTS: The method was tested on 115 clinical samples (81 koilocytic atypias, 11 CIN1, 10 CIN2, 12 CIN3 and 1 squamous carcinoma). HPV DNA was found in 56.7% koilocytic atypias, in 90.9% of CIN1 and in 100% of CIN2 and higher-grade lesions. Thus, PCR-RH is sensitive, rapid, easy-to-perform and readily applicable to the routine analysis of a large number of samples.
Предметные рубрики MeSH
BiopsyCarcinoma, Squamous CellCervix UteriDNA Probes, HPVDNA, ViralDigoxigeninEnzyme-Linked Immunosorbent AssayFemaleHumansNucleic Acid HybridizationPapillomavirus InfectionsPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthTumor Virus InfectionsUterine Cervical NeoplasmsUterine CervicitisUterine Cervical Dysplasia