Cutaneous psammomatous melanotic schwannoma: non-recurrence with surgical excision.

UNLABELLED: The present study examined the anti-inflammatory and functional improvement of the uterus and ovary, respectively, in bisphenol-A (BPA)-fed adult Wistar rats following the ingestion of methanolic extract of wheatgrass (WG-ME). Four groups of rats were conditioned as vehicle-treated control, BPA-treated (100 mg/kg b.w.), BPA + WG-ME (100 mg BPA/kg b.w. + 200 mg WG-ME/kg b.w.), and WG-ME (200 mg/kg b.w.) groups. The LC-MS study confirmed the presence of numerous bioactive components in WG-ME. ELISA, PAGE, real-time PCR, and immunohistostaining were executed to test the efficacy of WG-ME against BPA. WG-ME was shown to induce significant weight gain of the uterus and ovaries as well as improve the estrous cycle and antioxidant status. WG-ME effectively suppressed the mRNA expression of TNF-α (tumor necrosis factor-alpha) and NF-κB (nuclear factor kappa-B). This extract also increased the expression of the antiapoptotic factor BCL2 (B-cell lymphoma 2) in the uterine tissue of rats administered BPA while impeding the abnormal expression of the tumor proteins p53, cylcin-D1, and BAX (BCL2-associated protein X). An enhanced steroidogenic event was supported by improved gonadotropins and reproductive hormone levels, feeble signaling of androgen receptors, and improved ovarian follicular growth with a distinct appearance of granulosa layer as well as better uterine histomorphology. The abundance of apigenin and catechin compounds in WG-ME may potentiate the above effects. The molecular interaction study predicted that apigenin inhibits TNF-α by interacting with its major site. Hence, WG-ME may exert its preventive efficacy in managing the functional imbalance of reproductive organs caused by BPA.

SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-024-04117-0.