High-dimensional single-cell analysis evaluating effects of different airway management strategies on perioperative immune cells in non-small cell lung cancer surgery.

This study investigates the effects of different airway management strategies on perioperative immune cells in patients undergoing non-small cell lung cancer (NSCLC) surgery. A total of 23 patients were enrolled and randomly assigned to either the endotracheal intubation (ETT) group or the supraglottic airway device (SAD) group. Comprehensive mass cytometry analysis was performed on peripheral blood CD45+ cells to evaluate immune cell populations. The SAD group demonstrated significantly reduced consumption of neuromuscular blocking agents and opioid requirements compared to the ETT group, with comparable surgical parameters. Notably, the SAD group showed favorable trends in clinical recovery metrics such as analgesic requirements. The study highlights the impact of airway management techniques on immune function, suggesting that the SAD approach may better preserve immune cell populations during surgery. These findings contribute to understanding the early perioperative immune response in NSCLC patients and may inform clinical practices in anesthesia management.