Phytomedicine : international journal of phytotherapy and phytopharmacology · 2025
Gut-liver axis mechanisms of Hippophae rhamnoides L. in non-alcoholic fatty liver disease prevention.
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Ma Jing, Li Yujuan, Yang Yongjing, Zhao Linlin, Jiang Yan, Wong Tin Wui, Zhang Dejun
Аннотация
BACKGROUND: Phytosterol-rich Hippophae rhamnoides L. can ameliorate abnormal blood lipids in patients with non-alcoholic fatty liver disease (NAFLD). Its gut microbiota modulatory roles along gut-liver axis are not known.
OBJECTIVE: This study explored the preventive roles of H. rhamnoides L. against NAFLD and the associated gut-liver axis mechanisms.
METHODS: Sterols were extracted from H. rhamnoides L. seed oil (SHR) and subjected to LC-MS analysis. Their effects on body/liver/epididymal fat mass, intestinal microbiota, metabolites (DAO, D-LA, LPS) and SCFAs secretion, intestinal barrier (claudin-1, ZO-1) and liver lipid composition and molecular biology (AST, ALT, TC, TG, LDL-C, HDL-C, SREBP1c, SCD1, FAS, LXRα, PPARα) were examined in vivo against normal and high-fat diet mice. The modulatory action of microbiota on liver dysfunction was further verified through SHR-fed fecal transplantation into antibiotic-pretreated mice.
RESULTS: β-sitosterol, campesterol, stigmasterol, and ergosterol were the primary active constituents of SHR. With reference to high fat diet mice, SHR increased intestinal tight junction functionality, reducing inflammatory LPS migration to liver and hepatic lipid synthesis. SHR raised the relative abundance of Alloprevotella and Lachnospiraceae_NK4A136_group, and Bacteroides/Firmicutes ratio leading to increased SCFA secretion that strengthened intestinal barrier and reduced hepatic hyperlipidemia.
CONCLUSION: SHR effectively mitigated NAFLD by reducing hepatic lipid expression via regulating intestinal microbiota, its SCFA secretion and intestinal barrier.
OBJECTIVE: This study explored the preventive roles of H. rhamnoides L. against NAFLD and the associated gut-liver axis mechanisms.
METHODS: Sterols were extracted from H. rhamnoides L. seed oil (SHR) and subjected to LC-MS analysis. Their effects on body/liver/epididymal fat mass, intestinal microbiota, metabolites (DAO, D-LA, LPS) and SCFAs secretion, intestinal barrier (claudin-1, ZO-1) and liver lipid composition and molecular biology (AST, ALT, TC, TG, LDL-C, HDL-C, SREBP1c, SCD1, FAS, LXRα, PPARα) were examined in vivo against normal and high-fat diet mice. The modulatory action of microbiota on liver dysfunction was further verified through SHR-fed fecal transplantation into antibiotic-pretreated mice.
RESULTS: β-sitosterol, campesterol, stigmasterol, and ergosterol were the primary active constituents of SHR. With reference to high fat diet mice, SHR increased intestinal tight junction functionality, reducing inflammatory LPS migration to liver and hepatic lipid synthesis. SHR raised the relative abundance of Alloprevotella and Lachnospiraceae_NK4A136_group, and Bacteroides/Firmicutes ratio leading to increased SCFA secretion that strengthened intestinal barrier and reduced hepatic hyperlipidemia.
CONCLUSION: SHR effectively mitigated NAFLD by reducing hepatic lipid expression via regulating intestinal microbiota, its SCFA secretion and intestinal barrier.