Recombinant monoclonal antibodies for labeling tubulin post-translational modifications in Caenorhabditis elegans.

Post-translational modifications (PTMs) of tubulin regulate microtubule properties and functions. The Verhey lab created recombinant monoclonal antibodies (rMAbs) against tubulin acetylation (rMAb-6-11B-1), tyrosinated α-tubulin (rMAb-YL1/2), and glutamylation (rMAb-GT335) (Blasius et al., 2025; Hotta et al., 2026). Here, we validate these rMAbs in C. elegans hermaphrodites and males. These rMAbs faithfully reproduce the reported cell-type-specific staining patterns of commercial antibodies, providing high-quality, cost-effective resources for studying the tubulin code.