[Research on an improved Lowry method to determine content of protein in Sabin IPV].

OBJECTIVE

To establish a method for the content determination of protein in Sabin IPV.

METHODS

Using lowry method combined with being precipitated by trichloroacetic acid to determine the content of protein in Sabin IPV. Changing different conditions to optimize the experiment to establish a improved lowry method. And the sample recovery test was also conducted.

RESULTS

The method can exclude the interference of free aminoacid, phenols and some other additives. The calibration curve was in good linearity of protein within the range of 2.5 microg/ml-40 Microg/ml, r = 0.9998. Under the best conditions, the mean recovery was 95.32%, the CV in a batch and between batches were both < 10%.

CONCLUSION

The method can be used to determine the micro content of protein in vaccines.