Restorative dentistry & endodontics · 2026
Neuropeptide Y regulation of dental pulp neurogenic inflammation provoked by tooth bleaching agents: a descriptive comparative clinical study.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Caviedes-Bucheli Javier, Ríos-Osorio Néstor, Pérez-Villota Mario, Aucú-Miño Karolina, Escobar-Mafla Diana, Muñoz-Alvear Hernán Darío, Gomez-Sosa José Francisco, Diaz-Barrera Luis, Güiza-Cristancho Edgar, Munoz Hugo Roberto
Аннотация
OBJECTIVES: : This study aimed to assess the expression of neuropeptide Y (NPY) in human dental pulp after tooth bleaching with three in-office hydrogen peroxide (H2O2)-based systems.
METHODS: Forty pulps were collected from premolars scheduled for extraction and divided into four groups (n = 10): Control (no bleaching; basal NPY values); Pola Office (35% H2O2, 8 minutes); Opalescence Boost (40% H2O2, 20 minutes); and Zoom (25% H2O2 + cold blue light, 15 minutes). After extraction, pulps were fixed in 4% formaldehyde and processed. NPY levels were quantified using enzyme-linked immunosorbent assay. Data distribution was assessed with the Shapiro-Wilk test. One-way analysis of variance and Tukey post-hoc test with Bonferroni correction were applied (p < 0.05).
RESULTS: NPY expression differed significantly among groups (p = 0.0097). The control group showed the lowest mean expression (0.026 ± 0.002 pmol/mg of pulp tissue), followed by Zoom (0.031 ± 0.005 pmol/mg), Pola Office (0.040 ± 0.004 pmol/mg), and Opalescence Boost, which exhibited the highest NPY expression (0.044 ± 0.004 pmol/mg). Post-hoc analysis revealed a statistically significant difference between the control and Opalescence Boost groups (p = 0.0122).
CONCLUSIONS: The increase in NPY expression-particularly with Opalescence Boost-indicates that in-office bleaching agents can elicit measurable neurobiological responses in pulp tissue after a single application. The significant difference between the control and Opalescence Boost groups suggests a possible H2O2 concentration- or formulation-dependent effect on pulpal neuropeptide activity, underscoring the need for further research on the biological impact of bleaching treatments.
METHODS: Forty pulps were collected from premolars scheduled for extraction and divided into four groups (n = 10): Control (no bleaching; basal NPY values); Pola Office (35% H2O2, 8 minutes); Opalescence Boost (40% H2O2, 20 minutes); and Zoom (25% H2O2 + cold blue light, 15 minutes). After extraction, pulps were fixed in 4% formaldehyde and processed. NPY levels were quantified using enzyme-linked immunosorbent assay. Data distribution was assessed with the Shapiro-Wilk test. One-way analysis of variance and Tukey post-hoc test with Bonferroni correction were applied (p < 0.05).
RESULTS: NPY expression differed significantly among groups (p = 0.0097). The control group showed the lowest mean expression (0.026 ± 0.002 pmol/mg of pulp tissue), followed by Zoom (0.031 ± 0.005 pmol/mg), Pola Office (0.040 ± 0.004 pmol/mg), and Opalescence Boost, which exhibited the highest NPY expression (0.044 ± 0.004 pmol/mg). Post-hoc analysis revealed a statistically significant difference between the control and Opalescence Boost groups (p = 0.0122).
CONCLUSIONS: The increase in NPY expression-particularly with Opalescence Boost-indicates that in-office bleaching agents can elicit measurable neurobiological responses in pulp tissue after a single application. The significant difference between the control and Opalescence Boost groups suggests a possible H2O2 concentration- or formulation-dependent effect on pulpal neuropeptide activity, underscoring the need for further research on the biological impact of bleaching treatments.