Independent and combined effects of sepiolite and palygorskite on humus spectral properties and heavy metal bioavailability during chicken manure composting.

The effects of the independent and combined addition strategies of sepiolite and palygorskite on humification and heavy metals (HMs) during chicken manure composting were evaluated. Results showed that clay mineral addition showed a favorable effect on composting, prolonged the duration of the thermophilic phase (5-9 d) and improved the TN content (14%-38%) compared to CK. Independent strategy enhanced the humification degree in equal measures with the combined strategy. Carbon nuclear magnetic resonance spectroscopy (13C NMR) and fourier transform infrared spectroscopy (FTIR) confirmed that aromatic carbon species increased by 31%-33% during composting process. Excitation-emission matrix (EEM) fluorescence spectroscopy showed that humic acid-like compounds increased by 12%-15%. In addition, the maximum passivation rate of Cr, Mn, Cu, Zn, As, Cd, Pb and Ni were 51.35%, 35.98%, 30.39%, 32.46%, -87.02%, 36.61% and 27.62%, respectively. The independent addition of palygorskite exhibits the most potent effects for most HMs. Pearson correlation analysis indicated that pH and aromatic carbon were the key determinants of the HMs passivation. This study provided preliminary evidence and perspective of the application of clay minerals on the humification and safety of composting.