Journal of oral biosciences · 2026
ROS-scavenging and antibacterial properties of polydopamine-coated, drug-loaded bovine serum albumin nanoparticles.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Yang Libin, Li Yaoyang, Yang Guohua, Fu Ninghong, Ji Xiaoyuan, Guo Huihua, Chen Haijun, Cui Mengzhen, Chang Kexin, Liu Fuli, Jin Mengying, Li Rui, Bai Xiangyu, Wang Yuesheng
Аннотация
OBJECTIVES: Periodontitis is an inflammatory disease driven by dysbiotic dental plaque and is the leading cause of tooth loss in adults. We aimed to develop a reactive oxygen species (ROS)-responsive drug delivery system based on polydopamine (PDA)-functionalized bovine serum albumin (BSA) nanoparticles (NPs) for the controlled release of minocycline hydrochloride (MH) and to evaluate its synergistic antibacterial and antioxidant effects.
METHODS: MH-loaded BSA NPs were prepared using a desolventization method followed by PDA coating. The resulting PDA@BSA NPs@MH were characterized in terms of size, morphology, drug loading, and release kinetics. Antibacterial activity against Porphyromonas gingivalis, Streptococcus gordonii, and Fusobacterium nucleatum was assessed using live/dead staining, colony counting, and biofilm assays. ROS-scavenging activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and intracellular ROS assays. Biocompatibility was assessed via cytotoxicity, migration, and hemolysis tests.
RESULTS: PDA@BSA NPs@MH exhibited a uniform spherical morphology (180-200 nm) with 5.68% drug loading. The PDA coating enabled sustained MH release and concentration-dependent DPPH scavenging. The nanoparticles demonstrated potent antibacterial activity (mortality > 77% at high concentrations) and biofilm inhibition (>96%). Intracellular ROS levels were significantly reduced in LPS-stimulated macrophages. Furthermore, no evident cytotoxicity was observed, cell migration was enhanced, and hemolysis rates were <5%.
CONCLUSIONS: The PDA@BSA NPs@MH platform integrates MH-mediated antibacterial activity with PDA-mediated antioxidant activity, effectively addressing both bacterial infection and oxidative stress in periodontitis.
METHODS: MH-loaded BSA NPs were prepared using a desolventization method followed by PDA coating. The resulting PDA@BSA NPs@MH were characterized in terms of size, morphology, drug loading, and release kinetics. Antibacterial activity against Porphyromonas gingivalis, Streptococcus gordonii, and Fusobacterium nucleatum was assessed using live/dead staining, colony counting, and biofilm assays. ROS-scavenging activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and intracellular ROS assays. Biocompatibility was assessed via cytotoxicity, migration, and hemolysis tests.
RESULTS: PDA@BSA NPs@MH exhibited a uniform spherical morphology (180-200 nm) with 5.68% drug loading. The PDA coating enabled sustained MH release and concentration-dependent DPPH scavenging. The nanoparticles demonstrated potent antibacterial activity (mortality > 77% at high concentrations) and biofilm inhibition (>96%). Intracellular ROS levels were significantly reduced in LPS-stimulated macrophages. Furthermore, no evident cytotoxicity was observed, cell migration was enhanced, and hemolysis rates were <5%.
CONCLUSIONS: The PDA@BSA NPs@MH platform integrates MH-mediated antibacterial activity with PDA-mediated antioxidant activity, effectively addressing both bacterial infection and oxidative stress in periodontitis.