Potassium permanganate-hematite-modified biochar enhances cadmium and zinc passivation and nutrient availability and promotes soil microbial activity in heavy metal-contaminated soil.

Modified biochar has garnered considerable attention for its versatile applications in remediating soils contaminated with heavy metals. However, most existing studies have primarily focused on the stabilisation of heavy metals, with limited research exploring the broader environmental effects following the application of modified biochar. In this study, we developed a potassium permanganate (KMnO4)-hematite-modified biochar (MnFeB) as a passivating agent for heavy metals, specifically targeting cadmium (Cd) and zinc (Zn)-contaminated soils. We examined the effects of MnFeB on the biotoxicity of Cd and Zn, soil properties, enzyme activities, heavy metal resistance genes (czcA, czcC, and czcD), and the soil microbial community in contaminated soils. Treatment with MnFeB markedly reduced the soil diethylenetriaminepentaacetic acid (DTPA)-extractable Zn and Cd contents by 18.79% and 43.65%, respectively. Furthermore, soil organic carbon (SOC), cation exchange capacity (CEC), and the availability of nitrogen, phosphorus, and potassium were found to be increased. MnFeB application also enhanced the activities of catalase, urease, and alkaline phosphatase while reducing the expression of czcA by 23.63%. Moreover, changes in the composition and diversity of soil bacterial and fungal communities were observed. These findings highlight the effects of environmental changes induced by MnFeB application on Cd/Zn-contaminated soil and offer theoretical support for employing passivation strategies in the remediation of heavy metal-contaminated soils.