Lysosomal-targeted fluorescent probe for detecting SO2 derivatives in human umbilical vein endothelial cells.

A fluorescent probe (NBC), constructed by benzothiazole-coumarin and naphthalimide derivatives, was developed for the detection of SO2 derivatives using the FRET (Förster Resonance Energy Transfer) strategy. NBC presented large Stokes shift (180 nm), fast response (2 min), high sensitivity (LOD: 45 nM) and an excellent linear relationship in response to SO2 derivatives. Moreover, NBC has been successfully applied to detect SO2 derivatives in food samples and living cells. NBC holds significant potential for monitoring changes in both exogenous and endogenous SO2 derivatives in human umbilical vein endothelial cells (HUVEC cells). Additionally, it demonstrates remarkable lysosomal-targeting capabilities, allowing for the observation of dynamic alterations within lysosomes.