[Gas gangrene--case diagnosis].

Anaerobic spore-forming bacteria from species Clostridium perfringens is commonly present in soil and in the intestines of men and animals. Clostridium perfringens type A are responsible for gas gangrene men and animals. This paper describes gas gangrene case after limb amputation at 69-year-old man, treated for diabetes since several years. Used multiplex PCR generated amplification product 900 bp size (cpa) responsible for coding alpha toxin (CPA) present at Clostridium perfringens type A.