Infection and drug resistance · 2025
A New Composite Integrative and Conjugative Element Mediates Multiple Drug Resistance from Streptococcus anginosus to Streptococcus agalactiae.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Wang Yingting, Liu Taoran, Yi Sida, Zhu Yuanting
Аннотация
INTRODUCTION: Integrative and conjugative elements (ICEs) are a category of horizontal mobile genetic elements (MGEs) that play important roles in mediating the spread of antimicrobial resistance in Streptococci.
METHODS: In this study, a novel ICE, namely ICESan26_rplL, was identified from commensal Streptococcus anginosus through routine silico analyses. The genetic characterization of ICESan26_rplL was explored based on a comparison with known ICEs. The cyclization and cross-species transferability (from Streptococcus anginosus to Streptococcus agalactiae) of ICESan26_rplL were explored using inverse PCR and conjugation transfer experiments.
RESULTS: ICESan26_rplL is 61.618 kb in length at downstream of rplL and carries multiple antibiotic resistance genes, including erm(B) [for erythromycin, clindamycin, and streptococcin B (MLSB)], tetM-tetL (tetracycline), lnu and lsa(E) (clindamycin), and ant(6)-I (aminoglycosides). The comparative analysis results showed that ICESan26_rplL was a composite ICE with a mosaic structure composed of modules in the ICESa2603 and TnGBS families. The inverse PCR results demonstrated that ICESan26_rplL could be excised from the chromosome to form a circular intermediate. The conjugation transfer experiment and sequencing results confirmed the cross-species transfer of ICESan26_rplL to S. agalactiae recipients at a relatively low frequency (2.13 × 10-8). Moreover, core functional modules were retrieved from GenBank to search for any ICEs related to ICESan26_rplL. Eventually, 53 putative ICEs were identified, including three composite ICEs with high similarity to ICESan26_rplL, three ICEs in the TnGBS family, and 47 ICEs in the ICESa2603 family.
DISCUSSION: In this study, a novel mosaic ICE was reported for the first time. In addition, its transfer to the major pathogen Streptococcus agalactiae was characterized and proved. These findings suggest that this ICE is expected to become a vehicle for the dissemination of multiple antimicrobial resistance through the wider pathogen Streptococci.
METHODS: In this study, a novel ICE, namely ICESan26_rplL, was identified from commensal Streptococcus anginosus through routine silico analyses. The genetic characterization of ICESan26_rplL was explored based on a comparison with known ICEs. The cyclization and cross-species transferability (from Streptococcus anginosus to Streptococcus agalactiae) of ICESan26_rplL were explored using inverse PCR and conjugation transfer experiments.
RESULTS: ICESan26_rplL is 61.618 kb in length at downstream of rplL and carries multiple antibiotic resistance genes, including erm(B) [for erythromycin, clindamycin, and streptococcin B (MLSB)], tetM-tetL (tetracycline), lnu and lsa(E) (clindamycin), and ant(6)-I (aminoglycosides). The comparative analysis results showed that ICESan26_rplL was a composite ICE with a mosaic structure composed of modules in the ICESa2603 and TnGBS families. The inverse PCR results demonstrated that ICESan26_rplL could be excised from the chromosome to form a circular intermediate. The conjugation transfer experiment and sequencing results confirmed the cross-species transfer of ICESan26_rplL to S. agalactiae recipients at a relatively low frequency (2.13 × 10-8). Moreover, core functional modules were retrieved from GenBank to search for any ICEs related to ICESan26_rplL. Eventually, 53 putative ICEs were identified, including three composite ICEs with high similarity to ICESan26_rplL, three ICEs in the TnGBS family, and 47 ICEs in the ICESa2603 family.
DISCUSSION: In this study, a novel mosaic ICE was reported for the first time. In addition, its transfer to the major pathogen Streptococcus agalactiae was characterized and proved. These findings suggest that this ICE is expected to become a vehicle for the dissemination of multiple antimicrobial resistance through the wider pathogen Streptococci.