[Rapid simultaneous screening and detection of ten anticoagulant rodenticides in foods by ultra-high performance liquid chromatography-tandem mass spectrometry].

A rapid method for the simultaneous screening and detection of ten anticoagulant rodenticides in foods was developed by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After the extraction by acetonitrile and clean-up by QuEChERS, the extract was separated on a Poroshell 120 EC-C18 column (100 mm x 2.1 mm, 2.7 microm) with the gradient elution of 5 mmol/L ammonium acetate and acetonitrile. The detection was carried out by UPLC-MS/MS using a negative electrospray ionization interface in multiple reaction monitoring (MRM) mode. The ten anticoagulant rodenticides showed a good linear relationship (r > 0.99) in the range of 5 - 500 microg/L. In the four samples of chili sauce, flour, vinegar and soy sauce, the spiked recoveries were in the range of 72.6% - 112%, and the relative standard deviations (RSD) were all not more than 11.2%. The limits of detection (LOD) were all in the range of 0.5 - 4.5 microg/kg. This method is rapid, simple, sensitive, accurate and of good specificity for the satisfactory rapid screening and detection of the ten anticoagulant rodenticides in the sudden public health security events.