Muscle & nerve · 2014
Skin-derived precursor Schwann cell myelination capacity in focal tibial demyelination.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Grochmal Joey, Dhaliwal Sundeep, Stys Peter K, van Minnen Jan, Midha Rajiv
Аннотация
INTRODUCTION: Skin-derived precursor cells (SKPs) are neural crest progenitor cells that can attain a Schwann cell-like phenotype through in vitro techniques (SKP-SCs). We hypothesized that SKP-SCs could produce mature myelin and, in doing so, facilitate the recovery of a focal demyelination injury.
METHODS: We unilaterally injected DiI-labeled, green fluorescent protein (GFP)-producing SKP-SCs into the tibial nerves of 10 adult Lewis rats (with contralateral media control), 9 days after bilateral doxorubicin injury (0.38 μg). Tibial compound motor action potentials (CMAPs) were followed for 57 days. A separate morphometric cohort also included a Schwann cell injection group.
RESULTS: SKP-injected nerves recovered fastest in terms of electrophysiology and morphometry. SKP-SCs formed morphologically mature myelin, accounting for 15.3 ± 5.3% of the total myelin in SKP-SC-injected nerves.
CONCLUSIONS: SKP-SCs are robustly capable of myelination. They improve the recovery of a focal tibial nerve demyelination model by myelinating a measured percentage of axons.
METHODS: We unilaterally injected DiI-labeled, green fluorescent protein (GFP)-producing SKP-SCs into the tibial nerves of 10 adult Lewis rats (with contralateral media control), 9 days after bilateral doxorubicin injury (0.38 μg). Tibial compound motor action potentials (CMAPs) were followed for 57 days. A separate morphometric cohort also included a Schwann cell injection group.
RESULTS: SKP-injected nerves recovered fastest in terms of electrophysiology and morphometry. SKP-SCs formed morphologically mature myelin, accounting for 15.3 ± 5.3% of the total myelin in SKP-SC-injected nerves.
CONCLUSIONS: SKP-SCs are robustly capable of myelination. They improve the recovery of a focal tibial nerve demyelination model by myelinating a measured percentage of axons.
Предметные рубрики MeSH
Action PotentialsAnimalsAnimals, NewbornAntibiotics, AntineoplasticCells, CulturedDisease Models, AnimalDoxorubicinEvoked Potentials, MotorMaleNAV1.6 Voltage-Gated Sodium ChannelNeurofilament ProteinsPeripheral Blood Stem Cell TransplantationPolyradiculoneuropathyRanvier's NodesRatsRats, Inbred LewRecovery of FunctionSchwann CellsSkin