PloS one · 2014
GnRH agonist vs. hCG for triggering of ovulation--differential effects on gene expression in human granulosa cells.
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Haas Jigal, Ophir Libby, Barzilay Eran, Yerushalmi Gil M, Yung Yuval, Kedem Alon, Maman Ettie, Hourvitz Ariel
Аннотация
OBJECTIVE: To investigate the mRNA expression of genes related to steroidogenesis and OHSS in granulosa cells (GCs) of patients triggered with GnRH agonist compared to patients triggered with hCG.
DESIGN: Mural GCs were obtained at the time of oocyte retrieval and gene expression was analyzed using quantitative real time RT-PCR.
SETTINGS: Single center, case control study.
PATIENT(S): 24 women who were treated with GnRH agonist or hCG for triggering of ovulation.
INTERVENTIONS: GC collection.
MAIN OUTCOME MEASURE(S): The expression of genes related to steroidogenesis and OHSS in mural GCs.
RESULTS: The fertilization rate was similar in the two groups. The mRNA expression of CYP19A1 (0.50 vs 1, arbitrary unit), CYP11A1 (0.6 vs. 1) and 3 beta hydroxysteroid-dehydrogenase (0.39 vs 1) was significantly lower in the GnRH group. The expression of VEGF (0.74 vs. 1) and inhibin β B (0.38 vs 1) was lower in the GnRH analog triggered group.
CONCLUSION: Expression of genes related to steroidogenesis is lower at the time of oocyte retrieval in patients triggered with GnRH agonist. The decreased expression of VEGF and inhibin β B in the GnRH agonist group can explain the mechanism of early OHSS prevention.
DESIGN: Mural GCs were obtained at the time of oocyte retrieval and gene expression was analyzed using quantitative real time RT-PCR.
SETTINGS: Single center, case control study.
PATIENT(S): 24 women who were treated with GnRH agonist or hCG for triggering of ovulation.
INTERVENTIONS: GC collection.
MAIN OUTCOME MEASURE(S): The expression of genes related to steroidogenesis and OHSS in mural GCs.
RESULTS: The fertilization rate was similar in the two groups. The mRNA expression of CYP19A1 (0.50 vs 1, arbitrary unit), CYP11A1 (0.6 vs. 1) and 3 beta hydroxysteroid-dehydrogenase (0.39 vs 1) was significantly lower in the GnRH group. The expression of VEGF (0.74 vs. 1) and inhibin β B (0.38 vs 1) was lower in the GnRH analog triggered group.
CONCLUSION: Expression of genes related to steroidogenesis is lower at the time of oocyte retrieval in patients triggered with GnRH agonist. The decreased expression of VEGF and inhibin β B in the GnRH agonist group can explain the mechanism of early OHSS prevention.
Предметные рубрики MeSH
3-Hydroxysteroid DehydrogenasesAdultAromataseCase-Control StudiesCholesterol Side-Chain Cleavage EnzymeChorionic GonadotropinFemaleFertilization in VitroGene ExpressionGonadotropin-Releasing HormoneGranulosa CellsHumansInhibinsOocyte RetrievalOvarian Hyperstimulation SyndromeOvulationOvulation InductionPhosphoproteinsReceptors, LHReverse Transcriptase Polymerase Chain ReactionTriptorelin PamoateVascular Endothelial Growth Factor ASteroidogenic Acute Regulatory Protein