Annales pharmaceutiques francaises · 2026
Bioactive properties of Brachyotum naudinii Triana (Melastomataceae) leaves revealed by HPLC-ESI-QTOF-MS profiling and in vitro antioxidant and antibacterial assays.
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Aronés Jara Marco Rolando, Godoy Bautista Kirianova, Tôrres de Aquino Francisco José, Scalón Cunha Luis Carlos, Machado Martins Mário, Cárdenas Landeo Edgar, Cusinga Solano Eder, Paniagua Segovia Juan Clímaco, Conislla Cáceres Edith Eveling, Calla Choque Jaeson Santos, Rashid Anas, Aurora Prado María Segunda
Аннотация
BACKGROUND: Brachyotum naudinii Triana (Melastomataceae), a species widely distributed in the high Andean rocky outcrops of Ayacucho (Peru), has shown promising preliminary antioxidant and antimicrobial activity; however, its phytochemical composition and quantitative bioactivity remain poorly characterized.
OBJECTIVE: This study aimed to outline the complete profile of B. naudinii leaves phenolic constituents using accelerated solvent extraction (ASE) coupled to HPLC-ESI-QTOF-MS and to evaluate their in vitro antioxidant and antibacterial properties.
METHODS: Phenolic compounds were characterized using ASE-HPLC-ESI-QTOF-MS. Different extracts (hexane, ethyl acetate, and methanol) were evaluated for total phenolic and flavonoid contents, antioxidant activity (DPPH, ABTS, and FRAP assays), and antibacterial activity against Gram-positive and Gram-negative bacteria.
RESULTS: Twenty-four compounds were identified, predominantly ellagitannins, phenolic acids, and flavonol glycosides, confirming a polyphenol-rich profile. Among the tested extracts, the methanolic extract exhibited the highest total phenolic (496.3±2.75mg GAE/g extract) and flavonoid (101.0±0.67mg RUE/g extract) contents. Strong antioxidant activity was observed across all assays, with EC50 values of 41.0 ± 0.16μg/mL (DPPH), 123.5± 0.21 μg/mL (ABTS), and 41.3 ± 0.56μg/mL (FRAP), significantly lower than those of the reference antioxidant Trolox 100μg/mL (P<0.05). Antibacterial assays revealed inhibition zones ranging from 19.2 to 23.8mm, with Gram-positive bacteria being more susceptible than Gram-negative strains. Minimum inhibitory concentration values ranged from 6.28mg/mL for Staphylococcus aureus and Bacillus spizizenii to 50.10mg/mL for Escherichia coli and Salmonella typhimurium.
CONCLUSION: The observed bioactivities may be associated with the high polyphenolic content of the methanolic extract, although the contribution of individual compounds and potential synergistic effects were not experimentally evaluated. Overall, this study provides the first detailed chemical and functional characterization of B. naudinii leaves and supports their potential as a source of bioactive compounds with antioxidant and antibacterial relevance for nutraceutical and pharmacological applications.
OBJECTIVE: This study aimed to outline the complete profile of B. naudinii leaves phenolic constituents using accelerated solvent extraction (ASE) coupled to HPLC-ESI-QTOF-MS and to evaluate their in vitro antioxidant and antibacterial properties.
METHODS: Phenolic compounds were characterized using ASE-HPLC-ESI-QTOF-MS. Different extracts (hexane, ethyl acetate, and methanol) were evaluated for total phenolic and flavonoid contents, antioxidant activity (DPPH, ABTS, and FRAP assays), and antibacterial activity against Gram-positive and Gram-negative bacteria.
RESULTS: Twenty-four compounds were identified, predominantly ellagitannins, phenolic acids, and flavonol glycosides, confirming a polyphenol-rich profile. Among the tested extracts, the methanolic extract exhibited the highest total phenolic (496.3±2.75mg GAE/g extract) and flavonoid (101.0±0.67mg RUE/g extract) contents. Strong antioxidant activity was observed across all assays, with EC50 values of 41.0 ± 0.16μg/mL (DPPH), 123.5± 0.21 μg/mL (ABTS), and 41.3 ± 0.56μg/mL (FRAP), significantly lower than those of the reference antioxidant Trolox 100μg/mL (P<0.05). Antibacterial assays revealed inhibition zones ranging from 19.2 to 23.8mm, with Gram-positive bacteria being more susceptible than Gram-negative strains. Minimum inhibitory concentration values ranged from 6.28mg/mL for Staphylococcus aureus and Bacillus spizizenii to 50.10mg/mL for Escherichia coli and Salmonella typhimurium.
CONCLUSION: The observed bioactivities may be associated with the high polyphenolic content of the methanolic extract, although the contribution of individual compounds and potential synergistic effects were not experimentally evaluated. Overall, this study provides the first detailed chemical and functional characterization of B. naudinii leaves and supports their potential as a source of bioactive compounds with antioxidant and antibacterial relevance for nutraceutical and pharmacological applications.