Protocol for transcriptional profiling of fiber type and regeneration markers in mouse Tibialis Anterior muscle.

Skeletal muscle integrity and function depend on fiber-type composition and regenerative capacity. Evaluating both components in normal and injured tissues provides valuable insights into subtle declines in muscle function and recovery. We describe a protocol for the transcriptional analysis of the major fast-twitch type II myofibers and regeneration-related genes in normal and local-injured mouse Tibialis Anterior skeletal muscles. We describe steps for integrating freeze-induced injury, sample collection, tissue dissociation, RNA extraction, and gene expression analysis by quantitative reverse-transcription PCR (RT-qPCR).