Journal of ethnopharmacology · 2025
Phytochemical profiling and integrated in vitro/in vivo wound-healing evaluation of Barbeya oleoides Schweinfurth aerial parts.
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Ali Nermeen B, Adel Nagla, Avula Bharathi, Katragunta Kumar, Tatapudi Kiran Kumar, Khan Ikhlas A, Salama Abeer, Abdel-Sattar Essam, El-Shiekh Riham A
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Barbeya oleoides Schweinf. (Barbeyaceae) is traditionally employed to manage diverse ailments, including infections, fever, edema, and related inflammatory conditions.
AIM OF THE STUDY: This study aimed to investigate the wound-healing potential of the total methanolic extract (BO-TM) from the aerial parts of B. oleoides, as well as its defatted and mother liquor fractions (BO-ML). In addition, chemical profiling of identified bioactive constituents underpinning the observed therapeutic activity.
METHODS: LC-DAD-QToF analysis was performed for BO-TM phytochemical profiling. BO-TM was prepared and subsequently defatted to yield methylene chloride (BO-MC) and mother liquor (BO-ML) fractions. In vitro wound healing effects on human skin fibroblast (HSF) migration were assessed using a scratch assay, with effective doses selected based on prior MTT cytotoxicity assay results. For in vivo evaluation, male Wistar albino rats were divided into 7 groups: Normal, positive control without treatment, reference (Mebo®), and treatment groups receiving BO-TM and BO-ML (2.5 &5 % in Vaseline base, topically) once daily for 10 days. Histopathological examination and biochemical markers analyses (MIP-2, collagen, MDA, GSH, and FOXO1) were performed to elucidate underlying mechanisms related to inflammation, oxidative stress, and tissue remodeling.
RESULTS: Chemical profiling annotated 69 metabolites, comprising 20 flavonoids, 42 gallotannins and phenolic acid derivatives, and 5 triterpenoids. BO-TM (5 %) enhanced wound closure to 96 % versus 84 % in the untreated group. In vivo, treatment with BO-TM and BO-ML has achieved a significant increase in GSH, FOXO1, collagen, and MIP-2 levels while showing a significant decrease in MDA level compared to the positive control and Mebo® groups.
CONCLUSION: The methanolic extract and its fraction of B. oleoides exhibited significant-wound-healing potential in in vitro and in vivo models, likely through antioxidant, anti-inflammatory, and pro-regenerative mechanisms mediated by its rich polyphenolic and triterpenoid content. These findings provide scientific support for its traditional use and highlight its therapeutic potential as a natural wound-healing agent by Yemeni and Eritrean healers. However, further preclinical and clinical investigations are warranted to comprehensively evaluate its safety, efficacy, and translational potential in human applications.
AIM OF THE STUDY: This study aimed to investigate the wound-healing potential of the total methanolic extract (BO-TM) from the aerial parts of B. oleoides, as well as its defatted and mother liquor fractions (BO-ML). In addition, chemical profiling of identified bioactive constituents underpinning the observed therapeutic activity.
METHODS: LC-DAD-QToF analysis was performed for BO-TM phytochemical profiling. BO-TM was prepared and subsequently defatted to yield methylene chloride (BO-MC) and mother liquor (BO-ML) fractions. In vitro wound healing effects on human skin fibroblast (HSF) migration were assessed using a scratch assay, with effective doses selected based on prior MTT cytotoxicity assay results. For in vivo evaluation, male Wistar albino rats were divided into 7 groups: Normal, positive control without treatment, reference (Mebo®), and treatment groups receiving BO-TM and BO-ML (2.5 &5 % in Vaseline base, topically) once daily for 10 days. Histopathological examination and biochemical markers analyses (MIP-2, collagen, MDA, GSH, and FOXO1) were performed to elucidate underlying mechanisms related to inflammation, oxidative stress, and tissue remodeling.
RESULTS: Chemical profiling annotated 69 metabolites, comprising 20 flavonoids, 42 gallotannins and phenolic acid derivatives, and 5 triterpenoids. BO-TM (5 %) enhanced wound closure to 96 % versus 84 % in the untreated group. In vivo, treatment with BO-TM and BO-ML has achieved a significant increase in GSH, FOXO1, collagen, and MIP-2 levels while showing a significant decrease in MDA level compared to the positive control and Mebo® groups.
CONCLUSION: The methanolic extract and its fraction of B. oleoides exhibited significant-wound-healing potential in in vitro and in vivo models, likely through antioxidant, anti-inflammatory, and pro-regenerative mechanisms mediated by its rich polyphenolic and triterpenoid content. These findings provide scientific support for its traditional use and highlight its therapeutic potential as a natural wound-healing agent by Yemeni and Eritrean healers. However, further preclinical and clinical investigations are warranted to comprehensively evaluate its safety, efficacy, and translational potential in human applications.