A comparison between pyridinic and non-pyridinic extraction of allergenic materials.

The effect of addition of pyridine in the extracting solvent of allergenic materials was studied in the following substances. Dermatophagoides farinae, Chenopodium album, Parietaria judaica, Phleum pratense, Poa pratensis, and Secale cereale. Pyridinated extracts differed significantly in protein content, isoelectric focusing profile, and allergenic activity from the non-pyridinated extracts. On the other hand, no difference was detected between both extraction systems when their adsorption capacity to aluminium gels was compared.