International journal of radiation biology · 2026
Radiation protection of yeast, bacteria and V79 cells by various OH radical scavengers and a possible course of the protection.
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Múčka Viliam, Ondrák Lukáš, Čuba Václav, Neužilová Barbora
Аннотация
PURPOSE: The purpose of the paper was to investigate the radiation-protective effects of eight different scavengers on the Saccharomyces cerevisiae yeast, Escherichia coli bacteria and V79 cells inclusive the dependence of the specific protective effect on the efficiency of OH radicals scavenging and on the concentration of the scavenger under specific conditions.
MATERIALS AND METHODS: A suspension of yeast or bacteria in isotonic saline solution or V79 cells in phosphate buffer containing scavengers in various concentrations was irradiated with 60Co gamma radiation using different dose rates. The protective effects were evaluated from the surviving fractions after irradiation of the suspension of cells with and without the scavenger.
RESULTS: The slope of the linear dependence of protective effect on the scavenging efficiency is characteristic for a given scavenger and a given cell type. It was found to be surprisingly high at low concentrations of scavengers. The slope linearly increases with the dose rate for S. cerevisiae yeast protected with methanol or ethanol. But, this dependence is exponential for E. coli bacteria protected with methanol or ethanol and for V79 cells protected with DMSO. The slope was found to be a decreasing function of scavenging efficiency at a constant protective effect and increasing function of the scavenger concentration at constant scavenging efficiency.
CONCLUSIONS: The basis of the protective effect is the reaction of OH radicals with the scavenger. It is influenced by the mutual interaction of scavenger and OH radicals with a given cell type. The measurements at different dose rates allow to assume that this interaction is different in yeast than in bacteria or V 79 cells. Since the specific protective effect is extremely high in the range of low concentration of the scavenger, it can be assumed that several protection mechanisms may apply in this region.
MATERIALS AND METHODS: A suspension of yeast or bacteria in isotonic saline solution or V79 cells in phosphate buffer containing scavengers in various concentrations was irradiated with 60Co gamma radiation using different dose rates. The protective effects were evaluated from the surviving fractions after irradiation of the suspension of cells with and without the scavenger.
RESULTS: The slope of the linear dependence of protective effect on the scavenging efficiency is characteristic for a given scavenger and a given cell type. It was found to be surprisingly high at low concentrations of scavengers. The slope linearly increases with the dose rate for S. cerevisiae yeast protected with methanol or ethanol. But, this dependence is exponential for E. coli bacteria protected with methanol or ethanol and for V79 cells protected with DMSO. The slope was found to be a decreasing function of scavenging efficiency at a constant protective effect and increasing function of the scavenger concentration at constant scavenging efficiency.
CONCLUSIONS: The basis of the protective effect is the reaction of OH radicals with the scavenger. It is influenced by the mutual interaction of scavenger and OH radicals with a given cell type. The measurements at different dose rates allow to assume that this interaction is different in yeast than in bacteria or V 79 cells. Since the specific protective effect is extremely high in the range of low concentration of the scavenger, it can be assumed that several protection mechanisms may apply in this region.