Fertility and sterility · 2014
Sperm involved in recurrent partial hydatidiform moles cannot induce the normal pattern of calcium oscillations.
Клинический случайСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Nikiforaki Dimitra, Vanden Meerschaut Frauke, De Gheselle Stefanie, Qian Chen, Van den Abbeel Etienne, De Vos Winnok Harald, Deroo Tom, De Sutter Petra, Heindryckx Björn
Аннотация
OBJECTIVE: To assess the Ca2+-releasing ability of sperm involved in partial hydatidiform moles.
DESIGN: Analysis of the activating and Ca2+-releasing ability of human sperm.
SETTING: University hospital research laboratory.
PATIENT(S): Patients undergoing intracytoplasmic sperm injection (ICSI) treatment.
INTERVENTION(S): Microinjection of mouse and human oocytes with sperm.
MAIN OUTCOME MEASURE(S): Measurement of the fertilizing and Ca2+-releasing ability of human sperm.
RESULT(S): The mouse oocyte Ca2+ analysis showed that only 19.0% (4/21) of the mouse oocytes injected with sperm involved in molar pregnancies exhibited a normal pattern of Ca2+ oscillations versus 63.2% (36/57) of those injected with control sperm. Further, 83.3% (15/18) of donated in vitro-matured human oocytes injected with deficient sperm did not exhibit any Ca2+ release, while 76.9% (10/13) failed to show normal pronuclear development. Yet the sperm oocyte activation factor phospholipase C zeta (PLCζ) was present in the majority (96.6%, n=113) of the analyzed sperm at a normal expression level. Eventually, fertilization failure was overcome with assisted oocyte activation in subsequent therapeutic ICSI cycles, which led to normal deliveries.
CONCLUSION(S): Sperm that previously provoked recurrent partial hydatidiform mole pregnancies due to dispermic fertilization is not able to activate human oocytes or trigger the normal pattern of Ca2+ oscillations in mouse and human oocytes in vitro.
DESIGN: Analysis of the activating and Ca2+-releasing ability of human sperm.
SETTING: University hospital research laboratory.
PATIENT(S): Patients undergoing intracytoplasmic sperm injection (ICSI) treatment.
INTERVENTION(S): Microinjection of mouse and human oocytes with sperm.
MAIN OUTCOME MEASURE(S): Measurement of the fertilizing and Ca2+-releasing ability of human sperm.
RESULT(S): The mouse oocyte Ca2+ analysis showed that only 19.0% (4/21) of the mouse oocytes injected with sperm involved in molar pregnancies exhibited a normal pattern of Ca2+ oscillations versus 63.2% (36/57) of those injected with control sperm. Further, 83.3% (15/18) of donated in vitro-matured human oocytes injected with deficient sperm did not exhibit any Ca2+ release, while 76.9% (10/13) failed to show normal pronuclear development. Yet the sperm oocyte activation factor phospholipase C zeta (PLCζ) was present in the majority (96.6%, n=113) of the analyzed sperm at a normal expression level. Eventually, fertilization failure was overcome with assisted oocyte activation in subsequent therapeutic ICSI cycles, which led to normal deliveries.
CONCLUSION(S): Sperm that previously provoked recurrent partial hydatidiform mole pregnancies due to dispermic fertilization is not able to activate human oocytes or trigger the normal pattern of Ca2+ oscillations in mouse and human oocytes in vitro.