Phytomedicine : international journal of phytotherapy and phytopharmacology · 2026
Lipid-mediated broad-spectrum antiviral mechanism of alpha-linolenic acid against respiratory enveloped viruses.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Feng Yuan, Benma Lamu, Li Jiye, Shi Xunlong, Zhao Jing, Zeng Hulie, Tang Jianguo, Zhu Haiyan
Аннотация
BACKGROUND: α-Linolenic acid (ALA), an essential plant-derived polyunsaturated fatty acid with high safety, has been preliminarily shown to possess antiviral activity in vitro. However, the lack of in vivo activity evaluation and mechanism research has limited the understanding of its precise antiviral mechanism and therapeutic potential.
PURPOSE: This study elucidates the mechanism by which ALA disrupts viral lipid envelopes, conferring broad-spectrum antiviral activity, and evaluates its feasibility as a natural botanical agent against respiratory enveloped viruses.
METHODS: ALA (100 μM) was tested against H1N1, H3N2, and HCoV-229E in vitro. Its protective effect on the lungs were evaluated via intranasal and intraperitoneal administration in vivo. Raman spectroscopy, transmission electron microscopy, immunohistochemistry, and other techniques were employed to investigate the effects of ALA on viral envelopes, host membrane lipid signaling, pulmonary surfactant, lipoxygenases, and B-cell function. Additionally, a simulated mask application was tested to evaluate its practical antiviral potential.
RESULTS: ALA exhibited differential effects on viral and host membranes. Transmission electron microscopy and Raman spectroscopy confirmed that it disrupts viral envelopes while restoring the lipid balance of host membranes. Intranasal administration reduced viral loads and tissue damage in H1N1-infected mice, enhanced pulmonary surfactant and lipoxygenase levels independently of B-cell activation. ALA also showed broad-spectrum activity against HCoV-229E and H3N2, and further simulated mask tests confirmed that ALA significantly impaired viral infectivity.
CONCLUSION: This work establishes a unique dual mode of viral membrane destruction and host membrane protection for ALA. It innovatively reveals the lipid-targeted antiviral advantage of natural plant lipids, offering novel mechanistic insights and support for developing phytomedicine-based antiviral interventions.
PURPOSE: This study elucidates the mechanism by which ALA disrupts viral lipid envelopes, conferring broad-spectrum antiviral activity, and evaluates its feasibility as a natural botanical agent against respiratory enveloped viruses.
METHODS: ALA (100 μM) was tested against H1N1, H3N2, and HCoV-229E in vitro. Its protective effect on the lungs were evaluated via intranasal and intraperitoneal administration in vivo. Raman spectroscopy, transmission electron microscopy, immunohistochemistry, and other techniques were employed to investigate the effects of ALA on viral envelopes, host membrane lipid signaling, pulmonary surfactant, lipoxygenases, and B-cell function. Additionally, a simulated mask application was tested to evaluate its practical antiviral potential.
RESULTS: ALA exhibited differential effects on viral and host membranes. Transmission electron microscopy and Raman spectroscopy confirmed that it disrupts viral envelopes while restoring the lipid balance of host membranes. Intranasal administration reduced viral loads and tissue damage in H1N1-infected mice, enhanced pulmonary surfactant and lipoxygenase levels independently of B-cell activation. ALA also showed broad-spectrum activity against HCoV-229E and H3N2, and further simulated mask tests confirmed that ALA significantly impaired viral infectivity.
CONCLUSION: This work establishes a unique dual mode of viral membrane destruction and host membrane protection for ALA. It innovatively reveals the lipid-targeted antiviral advantage of natural plant lipids, offering novel mechanistic insights and support for developing phytomedicine-based antiviral interventions.