Zhonghua nei ke za zhi · 2001
[A study on the pathogenesis of Streptococcus mitis exotoxin].
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Lu H, Weng X, Yin Y, Chen Y, Jiang X, Wang H, Qian K, Zhu B, Peng B, Gong Z
Аннотация
OBJECTIVE: To study the isolation, purification and pathogenesis of Streptococcus mitis pyrogenic exotoxin causing toxic shock syndrome.
METHODS: Streptococcus mitis isolated from patients' throat were shaking cultivated. After being centrifuged, the supernatant fluid of the culture was precipitated with 20%, 40%, 60%, 80% (NH(4))(2)SO(4) respectively and the fast protein liquid chromatography(FPLC) was used for the final step of purification. Rabbits receiving subcutaneous injection with respective purified proteins were monitored daily for fever. The ability of the purified proteins to enhance the susceptibility of the rabbits to lethal Escherichia coli endotoxin shock is recorded, when the endotoxin was injected intravenously 4 hours after administration of 10 microg Streptococcus mitis exotoxin.
RESULTS: Only the protein precipitated by 20% (NH(4))(2)SO(4) (molecular weight is 34,000) from culture supernatant fluid was pyrogenic for rabbits (average temperature increase near 1 degrees C), and it can also cause the proliferation of rabbit splenocytes (mitogenicity). All the animals receiving subcutaneous injection of exotoxin containing purified proteins precipitated with higher concentrations of (NH(4))(2)SO(4) died within 16 approximately 29 hours after intravenous injection of the Escherichia coli endotoxin, demonstrating the enhanced susceptibility of the animals to lethal endotoxin shock. The control rabbits displayed none of these effects.
CONCLUSION: Streptococcus mitis exotoxin is a novel streptococcal pyrogenic exotoxin.
METHODS: Streptococcus mitis isolated from patients' throat were shaking cultivated. After being centrifuged, the supernatant fluid of the culture was precipitated with 20%, 40%, 60%, 80% (NH(4))(2)SO(4) respectively and the fast protein liquid chromatography(FPLC) was used for the final step of purification. Rabbits receiving subcutaneous injection with respective purified proteins were monitored daily for fever. The ability of the purified proteins to enhance the susceptibility of the rabbits to lethal Escherichia coli endotoxin shock is recorded, when the endotoxin was injected intravenously 4 hours after administration of 10 microg Streptococcus mitis exotoxin.
RESULTS: Only the protein precipitated by 20% (NH(4))(2)SO(4) (molecular weight is 34,000) from culture supernatant fluid was pyrogenic for rabbits (average temperature increase near 1 degrees C), and it can also cause the proliferation of rabbit splenocytes (mitogenicity). All the animals receiving subcutaneous injection of exotoxin containing purified proteins precipitated with higher concentrations of (NH(4))(2)SO(4) died within 16 approximately 29 hours after intravenous injection of the Escherichia coli endotoxin, demonstrating the enhanced susceptibility of the animals to lethal endotoxin shock. The control rabbits displayed none of these effects.
CONCLUSION: Streptococcus mitis exotoxin is a novel streptococcal pyrogenic exotoxin.