[The effect of Staphylococcus aureus toxic shock exotoxin on whole blood cell chemiluminescence in vitro and in vivo].

Stimulated and nonstimulated blood chemiluminescence were studied in presence of the toxic shock syndrome toxin I (TSST-I) in vivo and in vitro. In vivo experiments involved evaluation of rabbit whole blood chemiluminescence developed within various periods after the TSST-I intraperitoneal administration. Effect of various TSST-I concentrations on blood chemiluminescence was studied in vitro. TSST-I was shown to inhibit the nonstimulated chemiluminescence in blood within 45 min after administration of LD50 of both opsonized zymosan and calcium ionophore A 23187 in vivo. Similar effects the toxin exhibited at concentrations 20 microM and more in vitro. The maximum effect TSST-I demonstrated within the first 5 min of incubation and this effect was distinctly dose-dependent.