Biotechnology letters · 2026
Insight of Pichia kudriavzevii for single cell protein production and fermentation into ethanol using Thai banana as an alternative feedstock.
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Tawong Wittaya, Sujipuli Kawee, Boonsrangsom Thanita, Ratanasut Kumrop, Khieokhajonkhet Anurak, Khongnapha Woraphan, Rinrit Aphitchaya, Pongcharoen Pongsanat
Аннотация
PURPOSES: The objective of this study was to assess the potential of yeast Pichia kudriavzevii (NUNS-4, NUNS-5, NUNS-6, and TISTR5147) and Saccharomyces cerevisiae TISTR5606 strain to utilize banana, Musa ABB cv. (Kluai Namwa) and Musa sapientum L. cv. (Kluai Khai), biomass through the co-production of single cell protein and ethanol.
RESULTS: All tested strains grew well on both YPD and banana-based agar, confirming that banana residues can sustain yeast growth. Supplementation with banana biomass significantly enhanced protein and carbohydrate content. Notably, P. kudriavzevii TISTR5147, P. kudriavzevii NUNS-6, and S. cerevisiae TISTR5606 exhibited the highest single cell protein levels (50.85 ± 1.73%, 49.13 ± 1.50%, and 46.96 ± 1.07%, respectively) using Kluai Namwa, compared to controls without banana substrate. Ethanol yields were also increased when banana biomass was included. Co-fermentation of ripe pulp Kluai Khai with YPD resulted in the highest ethanol yields, reaching 77.63 ± 3.11 g/L for P. kudriavzevii NUNS-6, followed by 76.57 ± 4.49 g/L for NUNS-5, 74.17 ± 2.21 g/L for NUNS-4, and 72.32 ± 1.63 g/L for TISTR5147. Therefore, P. kudriavzevii NUNS-6 demonstrated superior efficiency as a dual producer of single-cell protein and ethanol.
CONCLUSION: These findings demonstrate that banana biomass is a cost-effective and sustainable carbon source for single-cell protein and ethanol production. Moreover, the P. kudriavzevii NUNS strains exhibited strong potential for bioconversion of banana residues into high-value products, supporting their applicability in future large-scale biotechnological processes.
RESULTS: All tested strains grew well on both YPD and banana-based agar, confirming that banana residues can sustain yeast growth. Supplementation with banana biomass significantly enhanced protein and carbohydrate content. Notably, P. kudriavzevii TISTR5147, P. kudriavzevii NUNS-6, and S. cerevisiae TISTR5606 exhibited the highest single cell protein levels (50.85 ± 1.73%, 49.13 ± 1.50%, and 46.96 ± 1.07%, respectively) using Kluai Namwa, compared to controls without banana substrate. Ethanol yields were also increased when banana biomass was included. Co-fermentation of ripe pulp Kluai Khai with YPD resulted in the highest ethanol yields, reaching 77.63 ± 3.11 g/L for P. kudriavzevii NUNS-6, followed by 76.57 ± 4.49 g/L for NUNS-5, 74.17 ± 2.21 g/L for NUNS-4, and 72.32 ± 1.63 g/L for TISTR5147. Therefore, P. kudriavzevii NUNS-6 demonstrated superior efficiency as a dual producer of single-cell protein and ethanol.
CONCLUSION: These findings demonstrate that banana biomass is a cost-effective and sustainable carbon source for single-cell protein and ethanol production. Moreover, the P. kudriavzevii NUNS strains exhibited strong potential for bioconversion of banana residues into high-value products, supporting their applicability in future large-scale biotechnological processes.