Enhanced antihypertensive chicken by-product hydrolysate fraction after its separation by electrodialysis with ultrafiltration membrane (EDUF).

The environmental impact of poultry industry waste has led to the study of hydrolysates with potential health-promoting properties obtained from poultry by-products and their fractionation to increase the bioactivity of these hydrolysates. The aim of the present study was to separate a chicken by-product hydrolysate (CBH) by electrodialysis with ultrafiltration membranes (EDUF), providing peptide selective separation based on their charge and molecular weight, and to characterize the resulting fractions. Experimental results showed that during the peptide fractionation process the global peptide migration rate (MR) from CBH was 14.97 ± 0.14 g/m2•h with a relative energy consumption of 31.27 ± 2.61 Wh/g of total peptides. 164 peptides were identified in the initial CBH, and following EDUF, 39 migrated to the positively charged peptide fraction (PCC) and 9 to the negatively charge peptide fraction (NCC): 21 sequences were reported as bioactive for CBH, 6 for PCC and 1 for NCC. Analyses of ACE inhibition evidenced a 1.4 fold increase in antihypertensive activity of the PCC (IC50 0.46 ± 0.04 mg peptides /mL) in comparison to CBH (IC50 0.65 ± 0. 04 mg peptides /mL), despite the smaller number of bioactive sequences reported and the fact that it is possible to enhance the PCC recovery by modifying the EDUF configuration in further studies. These findings highlight the significance of EDUF as a sustainable method for obtaining specifically charged peptide fractions with enhanced bioactivity from the initial hydrolysate.