Analytica chimica acta · 2025
Cu-N4 active site sensing platform for point-of-care colorimetric detection of environmental organophosphorus pesticide.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Huang Rong, Dong Zhong, Zhang Wanyi, Xue Zhenggang, Deng Dongmei, Wang Wei, Li Yuanyuan, He Haibo, Luo Liqiang
Аннотация
BACKGROUND: Organophosphorus pesticides contamination significantly threatens the ecological environment. Therefore, developing sensitive and efficient point-of-care detection methods is essential.
RESULTS: In this work, Cu-N4 single-atom nanozymes on nitrogen-doped carbon (Cu SAs/NC) with dual enzyme-mimicking activities (peroxidase-like and oxidase-like) were prepared for point-of-care detection of parathion using acetylcholinesterase inhibition-based colorimetric sensing mechanism. Cu SAs/NC shows high catalytic activity with high turnover number (Kcat) of ∼24.07 min-1 and Kcat/Km of ∼1.35 × 105 M-1 min-1. Experimental findings in combination with density functional theory simulations demonstrate that Cu centers in Cu SAs/NC play a pivotal role in intermediate binding optimization, which contributes to a decreased reaction energy barrier. Hence, a colorimetric sensing platform based on Cu SAs/NC was constructed for point-of-care detection of parathion, achieving a detection limit of 0.79 ng mL-1.
SIGNIFICANCE: This study provides a novel strategy for structuring single-atom nanozymes with enhanced enzyme-mimicking activity, showing great promise for advanced environmental protection.
RESULTS: In this work, Cu-N4 single-atom nanozymes on nitrogen-doped carbon (Cu SAs/NC) with dual enzyme-mimicking activities (peroxidase-like and oxidase-like) were prepared for point-of-care detection of parathion using acetylcholinesterase inhibition-based colorimetric sensing mechanism. Cu SAs/NC shows high catalytic activity with high turnover number (Kcat) of ∼24.07 min-1 and Kcat/Km of ∼1.35 × 105 M-1 min-1. Experimental findings in combination with density functional theory simulations demonstrate that Cu centers in Cu SAs/NC play a pivotal role in intermediate binding optimization, which contributes to a decreased reaction energy barrier. Hence, a colorimetric sensing platform based on Cu SAs/NC was constructed for point-of-care detection of parathion, achieving a detection limit of 0.79 ng mL-1.
SIGNIFICANCE: This study provides a novel strategy for structuring single-atom nanozymes with enhanced enzyme-mimicking activity, showing great promise for advanced environmental protection.