[Molecular epidemiological markers of Legionella pneumophila in the Valencian Community (II). Restriction enzyme profiles of chromosomal DNA].

Restriction enzyme profiles of chromosomal DNA obtained from 96 Legionella pneumophila strains isolated from environmental and clinical isolates and digested with Hind III have been analyzed. A method to obtain chromosomal DNA to be use in restriction studies and its electrophoretic analysis have been developed. Studying the fragments showing higher differences when comparing the profiles obtained, 5 subtypes are defined and correlated with the isolation areas. There are similar restriction profiles independent of the plasmid contents.