Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) · 2024
Salidroside prevents cadmium chloride-induced DNA damage in human fetal lung fibroblasts.
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Wu Yufei, Sun Chuan
Аннотация
BACKGROUND: Cadmium (Cd) is an environmental pollutant and a heavy metal known for its genotoxic effects, which can lead to cancer and other related diseases. Preventing Cd-induced genotoxicity is crucial; however, there is limited research on this topic. Salidroside (SAL), a phenylpropanoid glycoside isolated from Rhodiola rosea L., is a popular medicinal compound with several health benefits. Nevertheless, its therapeutic effect on Cd-induced genotoxicity remains unexplored.
METHODS: Human fetal lung fibroblasts were treated with 20 μM Cd2+ (CdCl2) for 12 h and 5-20 μM SAL was used to test the anti-DNA damage effect. DNA damage was evaluated using γH2AX expression and the alkaline comet assay. Intracellular reactive oxygen species (ROS) levels were measured using flow cytometry.
RESULTS: Exposure to 20 μM Cd2+ for 12 h induced significant DNA damage in human fetal lung fibroblasts, and this effect was notably attenuated by SAL treatment. SAL treatment did not decrease ROS levels in cells treated with Cd2+.
CONCLUSION: SAL effectively prevented Cd2+-induced DNA damage in human fetal lung fibroblasts. However, the underlying mechanism requires further investigation.
METHODS: Human fetal lung fibroblasts were treated with 20 μM Cd2+ (CdCl2) for 12 h and 5-20 μM SAL was used to test the anti-DNA damage effect. DNA damage was evaluated using γH2AX expression and the alkaline comet assay. Intracellular reactive oxygen species (ROS) levels were measured using flow cytometry.
RESULTS: Exposure to 20 μM Cd2+ for 12 h induced significant DNA damage in human fetal lung fibroblasts, and this effect was notably attenuated by SAL treatment. SAL treatment did not decrease ROS levels in cells treated with Cd2+.
CONCLUSION: SAL effectively prevented Cd2+-induced DNA damage in human fetal lung fibroblasts. However, the underlying mechanism requires further investigation.