A fluorescence spectrometry method for vitamin B6 determination based on its coordination reaction with Fe3.

In a pH 9.40 Clark-Lubs buffer solution, Vitamin B6 interacts with Fe3+, forming a coordination complex, increased the fluorescence signal of the reaction system. Based on the coordination reaction between Fe3+ and Vitamin B6, we proposed a novel method for determining Vitamin B6 content. The fluorescence intensity enhancements at 326 nm characteristic wavelength showed a linear relationship with Vitamin B6 concentrations in the range of 0.05-1.85 mg/L, with a correlation coefficient of 0.9966, and a detection limit of 0.0074 mg/L. This method was successfully applied in the determination of Vitamin B6 in tablet formulations. The proposed approach is simple, time-efffcient, cost-effective, and highly sensitive, thus suitable for the content detection of Vitamin B6.