Nature chemical biology · 2018
A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Chen Xue, Hagel Jillian M, Chang Limei, Tucker Joseph E, Shiigi Stacey A, Yelpaala Yuora, Chen Hsiang-Yun, Estrada Rodrigo, Colbeck Jeffrey, Enquist-Newman Maria, Ibáñez Ana B, Cottarel Guillaume, Vidanes Genevieve M, Facchini Peter J
Аннотация
The ultimate step in the formation of thebaine, a pentacyclic opiate alkaloid readily converted to the narcotic analgesics codeine and morphine in the opium poppy, has long been presumed to be a spontaneous reaction. We have detected and purified a novel enzyme from opium poppy latex that is capable of the efficient formation of thebaine from (7S)-salutaridinol 7-O-acetate at the expense of labile hydroxylated byproducts, which are preferentially produced by spontaneous allylic elimination. Remarkably, thebaine synthase (THS), a member of the pathogenesis-related 10 protein (PR10) superfamily, is encoded within a novel gene cluster in the opium poppy genome that also includes genes encoding the four biosynthetic enzymes immediately upstream. THS is a missing component that is crucial to the development of fermentation-based opiate production and dramatically improves thebaine yield in engineered yeast.