Analysis of specific interactions between influenza virus and cells of different sensitivity. Note 1. Analysis of influenza virus-specific RNA synthesis nonpermissive amniotic cells.

Virus-specific RNA synthesis was studied in amniotic AO cells nonpermissive for influenza virus. Syntheses of complementary RNA were found to be greatly reduced in AO cells, as compared with the sensitive MDCK cells. Only primary transcription takes place in AO cells, resulting in the production of poly-A RNAs serving as a template for the synthesis of virus-specific polypeptides. The synthesis of virion-type RNA is absent, since no synthesis of nonpoly-A transcripts (which are the templates for the synthesis of the respective RNA) could be made evident in the resistant AO cells.