Use of a DNA typing method to investigate a fatal perinatal infection due to Staphylococcus aureus.

The aim of this study was to determine, by random amplified polymorphic DNA analysis, the origin of a fatal Staphylococcus aureus perinatal infection which occurred after multiple cervical examinations before induction of labor for patient convenience. This DNA typing method was able to demonstrate that this infection originated from S. aureus genital carriage and urinary tract infection of the mother, and was not acquired in the hospital. This observation argues for the systematic screening of genital flora for highly virulent strains before induction of labor for non medical reasons. In addition, the DNA typing method used demonstrates that one neonate was subsequently infected by the strain responsible for the chorioamnionitis. This shows that molecular typing methods can help determine hospital staff responsibility.