Cascading microbial regulation of autochthonous DOM stability in a picocyanobacteria-dominated estuarine reservoir.

Estuaries serve as vital interfaces in the global carbon cycle by mediating land-ocean exchange and regulating dissolved organic matter (DOM) dynamics. However, the role of microbial communities in regulating autochthonous DOM under phosphorus-limited estuarine conditions remains insufficiently understood. This study explored the biogeochemical parameters, inorganic carbon dynamics, DOM optical properties, and algal-bacterial community composition in a picocyanobacteria-dominated estuarine reservoir subject to seasonal salinity and nutrient fluctuations. Samples were classified into three groups based on DOM compositional features: pristine autochthonous group (PG), high allochthonous group (HG), and balanced group (BG). In BG, picocyanobacteria, particularly Cyanobium PCC-6307, promoted the accumulation of labile tryptophan-like DOM (component C4), which was associated with the lowest autochthonous DOM stability ratios (AuSR). In HG, terrestrial runoff led to a decline in C4 and an increase in DOM stability, reflecting rapid microbial degradation and partial transformation. In BG, colder temperatures and elevated microbial α-diversity facilitated the conversion of DOM into more humified forms, as indicated by higher proportions of humic-like components and AuSR. Key microbial taxa showed substrate-specific metabolic traits related to amino acid, polysaccharide, and one-carbon compound processing. By integrating DOM-defined groupings, fluorescence-derived stability metrics, and microbial marker analysis, this study reveals a sequential cascade of microbial regulation in DOM production, transformation, and stabilization. These findings offer the first detailed evidence of such processes in a phosphorus-limited estuarine system and provide a new framework for linking DOM properties with microbial ecological functions in dynamic aquatic environments.