Open veterinary journal · 2025
Anticancer properties of ethanolic extract of Omani Ocimum basilicum in HCT116 colorectal cancer cells.
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Al-Mutaani Juma, Zorgui Tahani, Zourgui Lazhar, Alqawi Omar, Missaoui Nabiha
Аннотация
BACKGROUND: Natural plant-derived compounds are increasingly explored as safer alternatives to chemotherapy due to their multi-targeted anticancer properties. Despite Oman's rich floral biodiversity, data on the anticancer potential of its native species remain limited. Ocimum basilicum has emerged as a plant with promising bioactivities.
AIM: This study examined the cytotoxic, antimigratory, and antiadhesive effects of O. basilicum ethanolic extract on HCT116 colorectal cancer (CRC) cells.
METHODS: Ocimum basilicum collected from South Al Sharqiyah, Oman, was ethanol-extracted and tested against HCT116 cells at concentrations of 0-200 µg/ml. Cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, whereas cell adhesion and migration were assessed using fibrinogen/fibronectin-coated matrices and the wound-healing assay, respectively.Data were analyzed using one-way analysis of variance.
RESULTS: The ethanolic extract was rich in1,3-di-O-caffeoylquinic acid (23,366 ppm), rosmarinic acid (3,808 ppm), rutin (2,379 ppm), and luteolin (1,196 ppm).The extract significantly reduced HCT116 cell viability in a dose-dependent manner,from 87.7% at 50 µg/ml to 32.4% at 200 µg/ml (p < 0.0001). Migration was moderately inhibited at 50 µg/ml and strongly suppressed at concentrations ≥ 100 µg/ml (p < 0.0001), while adhesion remained unaffectedat 50 µg/ml but was significantly decreased at higher concentrations (p < 0.0001). A strong dose-response correlation was observed across all assays (R² > 0.89).
CONCLUSION: Ocimum basilicum ethanolic extract exhibits pronounced cytotoxic and antimigratory activities against HCT116 CRC cells, highlighting its potential as a natural anticancer candidate.
AIM: This study examined the cytotoxic, antimigratory, and antiadhesive effects of O. basilicum ethanolic extract on HCT116 colorectal cancer (CRC) cells.
METHODS: Ocimum basilicum collected from South Al Sharqiyah, Oman, was ethanol-extracted and tested against HCT116 cells at concentrations of 0-200 µg/ml. Cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, whereas cell adhesion and migration were assessed using fibrinogen/fibronectin-coated matrices and the wound-healing assay, respectively.Data were analyzed using one-way analysis of variance.
RESULTS: The ethanolic extract was rich in1,3-di-O-caffeoylquinic acid (23,366 ppm), rosmarinic acid (3,808 ppm), rutin (2,379 ppm), and luteolin (1,196 ppm).The extract significantly reduced HCT116 cell viability in a dose-dependent manner,from 87.7% at 50 µg/ml to 32.4% at 200 µg/ml (p < 0.0001). Migration was moderately inhibited at 50 µg/ml and strongly suppressed at concentrations ≥ 100 µg/ml (p < 0.0001), while adhesion remained unaffectedat 50 µg/ml but was significantly decreased at higher concentrations (p < 0.0001). A strong dose-response correlation was observed across all assays (R² > 0.89).
CONCLUSION: Ocimum basilicum ethanolic extract exhibits pronounced cytotoxic and antimigratory activities against HCT116 CRC cells, highlighting its potential as a natural anticancer candidate.