Toxicology mechanisms and methods · 2026
The mechanism of the PI3K/akt/NF-κB pathway in wasp venom-induced acute liver injury in rats: a preliminary study.
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Deng Fangfang, Chen Ronghui, Luo Wenxin, Liu Jie, Yang Guangli, Ma Lihong, Zhang Tianxi
Аннотация
OBJECTIVE: This study aims to preliminarily investigate the regulatory role of the PI3K/Akt/NF-κB signaling pathway in the process of wasp venom-induced acute liver injury.
METHODS: Rats were randomly divided into four groups using a random number table: Control group, PDTC group, ALI group, and ALI+PDTC group.
RESULTS: Significant changes were observed in the ALI group in the following aspects: (1) Behavioral Changes: Rats exhibited reduced activity and agitation 6 h after toxin exposure, with gradual relief observed after 24 h. (2) Biochemical Tests: The ALT and AST levels were significantly elevated at all time points (p < 0.05). (3) Pathological Changes: Liver cord structure disorganization, extensive necrosis, and neutrophil infiltration were observed, indicating time-dependent liver injury. (4) Inflammatory Cytokines: Levels of IL-6, TNF-α, and IFN-γ were significantly increased (p < 0.05), while IL-10 levels were decreased (p < 0.05). (5) Protein Expression: The expression of PI3K, Akt, and NF-κB p65 proteins was significantly elevated (p < 0.05). No significant differences were found between the Control and PDTC groups (p > 0.05), while the ALI+PDTC group showed notable relief.
CONCLUSION: This study successfully established a rat model of acute liver injury induced by wasp venom and found that the aberrant activation of the PI3K/Akt/NF-κB signaling pathway may contribute to liver injury progression by promoting inflammation. The NF-κB inhibitor PDTC significantly alleviated liver injury, an effect possibly associated with suppression of this pathway, providing a new theoretical basis for targeted treatment of secondary liver injury caused by clinical wasp stings.
METHODS: Rats were randomly divided into four groups using a random number table: Control group, PDTC group, ALI group, and ALI+PDTC group.
RESULTS: Significant changes were observed in the ALI group in the following aspects: (1) Behavioral Changes: Rats exhibited reduced activity and agitation 6 h after toxin exposure, with gradual relief observed after 24 h. (2) Biochemical Tests: The ALT and AST levels were significantly elevated at all time points (p < 0.05). (3) Pathological Changes: Liver cord structure disorganization, extensive necrosis, and neutrophil infiltration were observed, indicating time-dependent liver injury. (4) Inflammatory Cytokines: Levels of IL-6, TNF-α, and IFN-γ were significantly increased (p < 0.05), while IL-10 levels were decreased (p < 0.05). (5) Protein Expression: The expression of PI3K, Akt, and NF-κB p65 proteins was significantly elevated (p < 0.05). No significant differences were found between the Control and PDTC groups (p > 0.05), while the ALI+PDTC group showed notable relief.
CONCLUSION: This study successfully established a rat model of acute liver injury induced by wasp venom and found that the aberrant activation of the PI3K/Akt/NF-κB signaling pathway may contribute to liver injury progression by promoting inflammation. The NF-κB inhibitor PDTC significantly alleviated liver injury, an effect possibly associated with suppression of this pathway, providing a new theoretical basis for targeted treatment of secondary liver injury caused by clinical wasp stings.