Embryonic chromosomal abnormalities obtained after rescue intracytoplasmic sperm injection of 1-day-old unfertilized oocytes.

PURPOSE: To study if second day intracytoplasmic sperm injection (ICSI) results in chromosomal abnormalities in the embryos.

METHODS

Rescue ICSI was performed on 14 metaphase II (MII) oocytes after unsuccessful conventional IVF, four were fertilized. Fluorescent in situ hybridization (FISH) was performed on these four embryos and was informative for three.

RESULTS

There were two tetraploid embryos, one mosaic embryo with trisomy 21, tetrasomy 18, and tetrasomy for sex chromosomes in one cell and trisomy 22 in another cell.

CONCLUSIONS

We discourage the use of second day ICSI due to the observed increase in chromosomal abnormalities in these embryos.