Wei sheng yan jiu = Journal of hygiene research · 2007
[Study of the possible mechanism of effects of carbon disulfide on c-myc expression in rat testicles].
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Duan Yanying, Chen Guoyuan, Ji Jiajia, Lu Yan
Аннотация
OBJECTIVE: To survey the expression of c-myc of teticles in rats exposed to carbon disulfide (CS2) and the possible mechanism of effects on it.
METHODS: 36 wistar male rats were randomly divided into six groups. Four groups were exposed to CS2 at the dose of 0, 50, 250 and 1250mg/m3. The other two groups were treated at the dose of 1250 mg/ m3 CS2 plus VitE (250mg/kg diet) and VitE (250mg/kg diet) respectively. After 10-week esposures, rats were sacrificed and the SOD activity and MDA content and the c-myc expressions in testicles were determined.
RESULTS: When compared with the control group, SOD activities of testicles in the exposure group were decreased significantly (P < 0.01), MDA contents of testicles were increased significantly at the concentration of 1250mg/m3 CS2 (P < 0.01). The expression of c-myc of testicles present biphasic, and decreased at the concentraton of 50mg/m3 CS2 and increased at the other concentrations and higher than these of controls at the concentration of 1250mg/m3 CS2 significantly (P < 0.05). After treatment of CS2 combined with antioxidant VitE, SOD activity of testicles increased (P < 0.05), MDA content of testicles decreased (P < 0.01) and c-myc expression of testicles decreased consequently (P < 0.05).
CONCLUSION: the modulation of c-myc expression in testicles of rats treated with CS2 could be associated with lipid peroxidation induced by CS2.
METHODS: 36 wistar male rats were randomly divided into six groups. Four groups were exposed to CS2 at the dose of 0, 50, 250 and 1250mg/m3. The other two groups were treated at the dose of 1250 mg/ m3 CS2 plus VitE (250mg/kg diet) and VitE (250mg/kg diet) respectively. After 10-week esposures, rats were sacrificed and the SOD activity and MDA content and the c-myc expressions in testicles were determined.
RESULTS: When compared with the control group, SOD activities of testicles in the exposure group were decreased significantly (P < 0.01), MDA contents of testicles were increased significantly at the concentration of 1250mg/m3 CS2 (P < 0.01). The expression of c-myc of testicles present biphasic, and decreased at the concentraton of 50mg/m3 CS2 and increased at the other concentrations and higher than these of controls at the concentration of 1250mg/m3 CS2 significantly (P < 0.05). After treatment of CS2 combined with antioxidant VitE, SOD activity of testicles increased (P < 0.05), MDA content of testicles decreased (P < 0.01) and c-myc expression of testicles decreased consequently (P < 0.05).
CONCLUSION: the modulation of c-myc expression in testicles of rats treated with CS2 could be associated with lipid peroxidation induced by CS2.