Molecular insights into ANPEP in gastric adenocarcinoma.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Araújo Taíssa Maíra Thomaz, Rodrigues Bianca de Fátima Dos Reis, Silva Jessica Manoelli Costa da, Remígio Myrth Soares do Nascimento, Moreira Fabiano Cordeiro, Casseb Samir Mansour Moares, Barra Williams Fernandes, Ishak Geraldo, Anaissi Ana Karyssa Mendes, Magalhães Leandro, Vidal Amanda, Mourão Ronald Matheus da Silva, Teixeira Eliel Barbosa, Pereira Diego, Silva Valéria Cristiane Santos da, Avelar Daniel de Souza, Silva Rubem Ferreira, Santos Ândrea Kely Ribeiro Dos, Demachki Samia, Marques Livia Erika Carlos, Burbano Rommel Rodriguez, Assumpção Paulo Pimentel de
Аннотация
Alanyl aminopeptidase (ANPEP) has been implicated in various cancers, but its specific role in gastric adenocarcinoma (GC) remains incompletely understood. This study analyzed ANPEP gene expression in gastric cancer (GC), peritumoral tissue (PTT), metaplasia (M), and normal tissue (N). Total RNA was extracted, libraries were prepared and sequenced on the Illumina NextSeq 500. Data were processed using the nf-core/rnaseq pipeline. Transcript quantifications were imported with tximport and normalized using DESeq2. Differential expression (|log₂FC| > 1; adj. p < 0.05) and Kruskal-Wallis tests identified key genes. ANPEP was significantly upregulated in GC, PTT, and M compared to normal tissue (p < 0.01), suggesting its involvement in early mucosal transformation and malignant progression. Heatmap and pathway enrichment analysis revealed upregulation of genes related to immune function and oxidative stress, indicating an immunosuppressive and apoptosis-resistant tumor microenvironment. Correlation analyses identified strong positive associations between ANPEP and genes involved in cytoskeletal remodeling, immune modulation, and metabolic regulation, suggesting that ANPEP supports both the invasive potential of tumor cells and the establishment of an immunosuppressive, therapy-resistant niche. These findings position ANPEP as a promising biomarker for early detection and a candidate for targeted therapies.