Journal of ethnopharmacology · 2025
Millettia speciosa Champ. Leguminosae ameliorates testicular damage in mice by repairing of blood-testis barrier dysfunction and testosterone synthesis.
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Ai Qinglin, Cheng Yan, Lin Xiaoai, Zhang Ke, Zhou Ziyi, Deng Zhile, Fan Hao, Sun Yongxue, Lei Wei, Liang Xiaohuan
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Millettia speciosa Champ. Leguminosae (MSC), a medicinal-edible plant in southern China, according to the records in Lingnan caiyaolu, MSC is known for its functions of "tonifying deficiency and consolidating essence." It is also empirically used to enhance male reproductive health. However, the protective mechanisms of MSC on the testes require further investigation.
AIM OF THE STUDY: This study aims to investigate the protective effect of MSC extract against cyclophosphamide (CP)-induced testicular damage.
METHODS: The components of MSC were conducted using ultra-performance liquid chromatography combined with UPLC-ESI-MS/MS. Testicular dysfunction was induced in ICR mice by intraperitoneal injection of CP (50 mg/kg, once a day for one week), and MSC (400 or 800 mg/kg) was administered orally daily for four weeks to evaluate its effectiveness in alleviating CP-induced testicular dysfunction. Sperm quality was assessed by computer-assisted semen analysis (CASA), hematoxylin-eosin (H&E) staining, immunohistochemistry (IHC) immunofluorescence (IF) and in situ hybridization (ISH) were conducted to analyze the recovery to testicular function. Quantitative analysis of ROS indicators and hormones was performed using enzyme-linked immunosorbent assay (ELISA). The expressions of related genes were evaluated by Quantitative real-time PCR (RT-qPCR).
RESULTS: MSC alleviated CP-induced reductions in body weight, testicular index, and epididymal index. It enhanced antioxidant enzyme activity such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and upregulated related genes (Sod1, Sod2, Sod3, and Cat). MSC also improved sperm quality, restored testosterone (T) levels by upregulating genes involved in T synthesis (Cyp17a1, Cyp11a1, and Star), and reinforced the blood-testis barrier (BTB) through increased expression of Zonula occludens-1, Connexin43, and N-Cadherin. Notably, MSC reinitiated the stalled meiotic process, as evidenced by restored expression of Stra8, and the reappearance of spermatogenic cells in the seminiferous tubules and their correct localization.
CONCLUSION: This study demonstrates the anti-oxidative stress capability of MSC and highlights its multifaceted protective mechanisms against CP-induced testicular damage, suggesting its potential as a phytotherapeutic treatment for testicular damage.
AIM OF THE STUDY: This study aims to investigate the protective effect of MSC extract against cyclophosphamide (CP)-induced testicular damage.
METHODS: The components of MSC were conducted using ultra-performance liquid chromatography combined with UPLC-ESI-MS/MS. Testicular dysfunction was induced in ICR mice by intraperitoneal injection of CP (50 mg/kg, once a day for one week), and MSC (400 or 800 mg/kg) was administered orally daily for four weeks to evaluate its effectiveness in alleviating CP-induced testicular dysfunction. Sperm quality was assessed by computer-assisted semen analysis (CASA), hematoxylin-eosin (H&E) staining, immunohistochemistry (IHC) immunofluorescence (IF) and in situ hybridization (ISH) were conducted to analyze the recovery to testicular function. Quantitative analysis of ROS indicators and hormones was performed using enzyme-linked immunosorbent assay (ELISA). The expressions of related genes were evaluated by Quantitative real-time PCR (RT-qPCR).
RESULTS: MSC alleviated CP-induced reductions in body weight, testicular index, and epididymal index. It enhanced antioxidant enzyme activity such as superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and upregulated related genes (Sod1, Sod2, Sod3, and Cat). MSC also improved sperm quality, restored testosterone (T) levels by upregulating genes involved in T synthesis (Cyp17a1, Cyp11a1, and Star), and reinforced the blood-testis barrier (BTB) through increased expression of Zonula occludens-1, Connexin43, and N-Cadherin. Notably, MSC reinitiated the stalled meiotic process, as evidenced by restored expression of Stra8, and the reappearance of spermatogenic cells in the seminiferous tubules and their correct localization.
CONCLUSION: This study demonstrates the anti-oxidative stress capability of MSC and highlights its multifaceted protective mechanisms against CP-induced testicular damage, suggesting its potential as a phytotherapeutic treatment for testicular damage.