Analytica chimica acta · 2025
Preparation of copper-based protein-inorganic hybrid nanoflowers with peroxidase-like activity for colorimetric detection of biothiols.
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Li Hongyu, Cen Li, Liu Shiyu, Qiu Ziqing, Zhang Yanfei, Luo Xingyu
Аннотация
BACKGROUND: Biothiols play significant roles in maintaining cellular redox balance detoxification, signal transduction, and protein structure stabilization. Developing detection methods for biothiols analysis has gradually become an effective approach to monitor their contents fluctuation and evaluate these relative health problems in humans. Although conventional methods for biothiols analysis have been designed, it still remains the limitation of bulky-instrument requirements, inconvenience and inefficiency in practical measurement. Hence, there is an urgent demand to develop efficient detection methods for monitoring biothiols in real scenarios.
RESULTS: By virtue of the highly stable and efficient peroxidase (POD)-like activity of nanozymes, we here propose a nanozyme-based visual detection method that rationally integrated the facile synthesized BSA-Cu3(PO4)2 HNFs with a smartphone detection platform for proceeding visual and portable detection performances. In detail, we primarily utilize the bovine serum albumin (BSA) containing abundant amine and amide groups as the organic ligand and Cu3(PO4)2 as the inorganic active component to successfully prepare BSA-Cu3(PO4)2 HNFs, and then biothiols can be served as the competitors for disturbing the colorimetric signals produced by BSA-Cu3(PO4)2 HNFs-triggered peroxidization in the TMB-H2O2 system. Taking advantages of the favorable catalytic activity of BSA-Cu3(PO4)2 HNFs, it enables a sensitive detection for biothiols including glutathione (GSH), cysteine (Cys), and homocysteine (Hcy). The proposed method has presented good detection performances of biothiols with linear relationships ranging from 10 to 200 μM, and the corresponding limits of detection (LODs) of GSH, Cys, and Hcy were calculated to be 5.4 μM, 3.5 μM, and 7.7 μM, respectively.
SIGNIFICANCE: This proposed method has also achieved a convenient detection performance to realize biothiols analysis in real scenarios. The colorimetric signals of biothiols can be transformed into RGB values by a smartphone-based detection platform, indicating the great potential in facile, sensitive, and instrument-free analysis in monitoring biothiols contents variation.
RESULTS: By virtue of the highly stable and efficient peroxidase (POD)-like activity of nanozymes, we here propose a nanozyme-based visual detection method that rationally integrated the facile synthesized BSA-Cu3(PO4)2 HNFs with a smartphone detection platform for proceeding visual and portable detection performances. In detail, we primarily utilize the bovine serum albumin (BSA) containing abundant amine and amide groups as the organic ligand and Cu3(PO4)2 as the inorganic active component to successfully prepare BSA-Cu3(PO4)2 HNFs, and then biothiols can be served as the competitors for disturbing the colorimetric signals produced by BSA-Cu3(PO4)2 HNFs-triggered peroxidization in the TMB-H2O2 system. Taking advantages of the favorable catalytic activity of BSA-Cu3(PO4)2 HNFs, it enables a sensitive detection for biothiols including glutathione (GSH), cysteine (Cys), and homocysteine (Hcy). The proposed method has presented good detection performances of biothiols with linear relationships ranging from 10 to 200 μM, and the corresponding limits of detection (LODs) of GSH, Cys, and Hcy were calculated to be 5.4 μM, 3.5 μM, and 7.7 μM, respectively.
SIGNIFICANCE: This proposed method has also achieved a convenient detection performance to realize biothiols analysis in real scenarios. The colorimetric signals of biothiols can be transformed into RGB values by a smartphone-based detection platform, indicating the great potential in facile, sensitive, and instrument-free analysis in monitoring biothiols contents variation.