Molecular biology reports · 2026
Biofilm disruption by citronellol and geraniol against clinical uropathogenic Pseudomonas aeruginosa: an integrated in vitro and in silico investigation.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Benaissa Asma, Tamfu Alfred Ngenge, Bouali Waffa, Bousselham Ammara, Medjdoub Houria, Babali Brahim, Busra Arslan Eroglu, Kucukaydin Selcuk, Anouar El-Hassane, Ceylan Ozgur
Аннотация
BACKGROUND: Antimicrobial resistance (AMR) by Pseudomonas aeruginosa, within urinary tract and nosocomial infections, involves biofilm formation and quorum sensing (QS) constituting a major clinical challenge.
METHODS: The chemical composition of Pelargonium graveolens essential oil (EO) was determined by gas chromatography-mass spectrometry (GC-MS). Antibacterial activity of the EO and its major constituents, citronellol and geraniol, was evaluated against clinical and reference P. aeruginosa strains using disk diffusion and broth microdilution assays. Antibiofilm activity was assessed at concentrations ranging from 2×MIC to sub-MIC levels. Interference with quorum-sensing-associated phenotypes was investigated using Chromobacterium violaceum CV12472 and CV026 biosensor strains. Swarming and swimming motility assays were performed. Molecular docking and ADMET analyses were conducted to explore potential target interactions and pharmacokinetic properties.
RESULTS: Citronellol (34.29%) and geraniol (18.62%) were the predominant constituents of the EO. Antibacterial activity yielded inhibition zones of 10-14 mm and MIC values ranging from 1% to 0.031% (v/v), with geraniol showing the lowest MIC (0.031%). The EO, citronellol, and geraniol inhibited biofilm formation, reaching 87.28% inhibition at 2×MIC while retaining activity at sub-MIC concentrations. All treatments reduced violacein production in C. violaceum biosensors and inhibited bacterial motility, with maximum reductions of 82.6% (swarming) and 74.3% (swimming). Geraniol generally exhibited the strongest activity. Docking analysis revealed binding affinities of - 6.08 and - 5.83 kcal/mol for citronellol and geraniol, respectively.
CONCLUSION: P. graveolens EO, citronellol, and geraniol exhibited promising anti-virulence properties against multidrug-resistant uropathogenic P. aeruginosa, supporting their potential as complementary agents for controlling biofilm- and motility-associated infections.
METHODS: The chemical composition of Pelargonium graveolens essential oil (EO) was determined by gas chromatography-mass spectrometry (GC-MS). Antibacterial activity of the EO and its major constituents, citronellol and geraniol, was evaluated against clinical and reference P. aeruginosa strains using disk diffusion and broth microdilution assays. Antibiofilm activity was assessed at concentrations ranging from 2×MIC to sub-MIC levels. Interference with quorum-sensing-associated phenotypes was investigated using Chromobacterium violaceum CV12472 and CV026 biosensor strains. Swarming and swimming motility assays were performed. Molecular docking and ADMET analyses were conducted to explore potential target interactions and pharmacokinetic properties.
RESULTS: Citronellol (34.29%) and geraniol (18.62%) were the predominant constituents of the EO. Antibacterial activity yielded inhibition zones of 10-14 mm and MIC values ranging from 1% to 0.031% (v/v), with geraniol showing the lowest MIC (0.031%). The EO, citronellol, and geraniol inhibited biofilm formation, reaching 87.28% inhibition at 2×MIC while retaining activity at sub-MIC concentrations. All treatments reduced violacein production in C. violaceum biosensors and inhibited bacterial motility, with maximum reductions of 82.6% (swarming) and 74.3% (swimming). Geraniol generally exhibited the strongest activity. Docking analysis revealed binding affinities of - 6.08 and - 5.83 kcal/mol for citronellol and geraniol, respectively.
CONCLUSION: P. graveolens EO, citronellol, and geraniol exhibited promising anti-virulence properties against multidrug-resistant uropathogenic P. aeruginosa, supporting their potential as complementary agents for controlling biofilm- and motility-associated infections.