Heptaminol-induced metabolic liver and cardiac injuries in rats: phytochemical screening, experimental, computational modelling and pharmacological study of phoenix dactylifera seeds.

Introduction

Heptaminol (HEP) is widely used and characterized by liver and cardiac risk factors, dysregulated prooxidant-antioxidant balance, and inflammation. This study aimed to study the phytochemical composition of date seeds of Phoenix dactylifera (DSPE) by GC-MS analysis and to assess the in vitro antioxidant, anticoagulant and ACE activities.

Methods

The hepato and cardiotoprotective effect against HEP-induced toxicity in rats have been assessed using biochemical, histological and computational assays.

Results

HEP increased the body weight, associated significant increases in total cholesterol, triglycerides, total and direct bilirubin, alkaline phosphatase, creatinine kinase-MB, ACE, and troponin-T, exhibited changes in ECG pattern, including ST-segment elevation, and increased rate of TBARS with decreases in the antioxidant enzymatic. DSPE improved these biomarkers alterations through anti-oxidative and anti-coagulant activities, which were confirmed by histopathological examinations. The computational findings supported the in vivo results and showed that DSPE compounds bound Hypoxia-Inducible Factor (HIF-1α) and Insulin-Like Growth Factor (IGF1) with acceptable affinities and molecular interactions.

Conclusion

DSPE had hepato and cardioprotective effects against HEP-induced heart and liver injuries. DSPE can be used to prevent acute thrombosis due to its different bioactive compounds.