[Research of chromosome aberration in Chinese hamster lung fibroblast induced by VEGF siRNA].

OBJECTIVE

To explore the effects of VEGF siRNA on chromosome aberration in Chinese hamster lung fibroblasts (CHL).

METHODS

The chromosome aberrations were observed after CHL were transfected with VEGF siRNA for 24 hours and 48h.

RESULTS

There were a questionable positive in 100nmol/L VEGF siRNA after VEGF siRNA were transfected for 24h and the chromosome aberration rate was 6 percentages. Both 50nmol/L VEGF siRNA and 100nmol/L VEGF siRNA caused the questionable positive after VEGF siRNAs were transfected for 48h, and the chromosome aberration rates was 6 percentages and 10 percentages separately. There were no the chromosome aberration in 25nmol/L VEGF siRNA. The types of chromosomal aberration induced by VEGF siRNAs included break, Dicentric grain, polyploid, gap, and three trajectories.

CONCLUSION

100nmol/L VEGF siRNA might cause CHL chromosome aberration.