Effects of diazepam and cyclohexyladenosine on open-field behavior in rats perinatally exposed to caffeine.

Adult rat offspring earlier exposed to maternally ingested caffeine during both gestation and lactation were observed in an open field following acute administration of diazepam or cyclohexyladenosine. While both drugs reduced measures of locomotion and emotional reactivity, caffeine-exposed rats showed evidence of greater sensitivity to cyclohexyladenosine (but not diazepam) as determined by its effects on grooming behavior and tendencies to occupy the center squares of the apparatus. This suggested that adenosine (A1) rather than benzodiazepine receptor activity had been affected by the perinatal caffeine experience which also reduced locomotor activity while increasing center occupancy. The acute effects of diazepam and cyclohexyladenosine also depended largely on the sex of the subjects. Diazepam affected locomotor activity more and both drugs affected defecation less in females than in males. No other interaction involving sex was significant.