Medicine and science in sports and exercise · 2025
The Effect of Resistance Training and Androgen Administration on Neuromuscular Junction Morphology and Neurotrophin Expression.
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Ben-Zeev Tavor, Levi Chagai, Hoffman Jay R
Аннотация
PURPOSE: This study examined the effect of resistance training (RT) and nandrolone decanoate (ND) administration on neuromuscular junction (NMJ) morphology and neurotrophin expression.
METHODS: Forty 3-month-old male C57Bl/6J mice were randomized to an RT or sedentary (SED) group. Each group of animals was further divided into sham (S) or ND (35 mg·kg -1 ·wk -1 ) groups. All interventions were 7 wk in duration. Brain-derived neurotrophic factor (BDNF), tropomyosin receptor kinase B (TrkB), p75, and androgen receptor (AR) expression in the plantaris and soleus muscles were examined via Western blot, whereas NMJ morphology was examined via immunofluorescence.
RESULTS: Significant interactions for BDNF expression were noted in the plantaris ( P = 0.009) and soleus ( P = 0.010). In the plantaris BDNF expression for RT-ND was greater than RT-S ( P = 0.019) and SED-ND ( P = 0.016), whereas BDNF expression in RT-S was greater than all other groups ( P 's < 0.05) in the soleus. A significant interaction for TrkB expression was observed in the plantaris. TrkB in RT-ND was significantly greater ( P = 0.031) than SED-ND. Analysis of the postsynaptic membrane within the plantaris revealed that RT increased the acetylcholine (ACh) receptor-stained area ( P = 0.036), ACh-stained perimeter ( P = 0.006), and total area ( P = 0.044) compared with SED. In addition, ND administration increased ( P = 0.050) ACh total area. In the presynaptic membrane, both RT and ND increased ACh vesicle-stained area ( P < 0.001 and P = 0.006, respectively), ACh vesicle-stained perimeter ( P < 0.001 and P = 0.02, respectively), and ACh vesicle total area ( P < 0.001 and P = 0.01, respectively), whereas RT only was noted to increase presynaptic ACh vesicles total perimeter ( P < 0.001). In the soleus, ND increased ACh receptor-stained ( P = 0.002) and total area ( P = 0.035) at the post-synaptic membrane.
CONCLUSIONS: RT and ND administrations stimulate increases in neurotrophin expression and NMJ morphology in plantaris and soleus muscles.
METHODS: Forty 3-month-old male C57Bl/6J mice were randomized to an RT or sedentary (SED) group. Each group of animals was further divided into sham (S) or ND (35 mg·kg -1 ·wk -1 ) groups. All interventions were 7 wk in duration. Brain-derived neurotrophic factor (BDNF), tropomyosin receptor kinase B (TrkB), p75, and androgen receptor (AR) expression in the plantaris and soleus muscles were examined via Western blot, whereas NMJ morphology was examined via immunofluorescence.
RESULTS: Significant interactions for BDNF expression were noted in the plantaris ( P = 0.009) and soleus ( P = 0.010). In the plantaris BDNF expression for RT-ND was greater than RT-S ( P = 0.019) and SED-ND ( P = 0.016), whereas BDNF expression in RT-S was greater than all other groups ( P 's < 0.05) in the soleus. A significant interaction for TrkB expression was observed in the plantaris. TrkB in RT-ND was significantly greater ( P = 0.031) than SED-ND. Analysis of the postsynaptic membrane within the plantaris revealed that RT increased the acetylcholine (ACh) receptor-stained area ( P = 0.036), ACh-stained perimeter ( P = 0.006), and total area ( P = 0.044) compared with SED. In addition, ND administration increased ( P = 0.050) ACh total area. In the presynaptic membrane, both RT and ND increased ACh vesicle-stained area ( P < 0.001 and P = 0.006, respectively), ACh vesicle-stained perimeter ( P < 0.001 and P = 0.02, respectively), and ACh vesicle total area ( P < 0.001 and P = 0.01, respectively), whereas RT only was noted to increase presynaptic ACh vesicles total perimeter ( P < 0.001). In the soleus, ND increased ACh receptor-stained ( P = 0.002) and total area ( P = 0.035) at the post-synaptic membrane.
CONCLUSIONS: RT and ND administrations stimulate increases in neurotrophin expression and NMJ morphology in plantaris and soleus muscles.