FT-IR and GC-MS analyses of potential bioactive compounds of cow urine and its antibacterial activity.

The main emphasis of this study was to identify the bioactive compounds responsible for antibacterial activity of Badri cow urine isolated by thin layer chromatography. The most effective bioactive fraction was analysed by FT-IR and GC-MS analyses. Among the four major fractions (EW1, EW2, CA1 and CA2) obtained by TLC profiling, EW1 was found most active against bacterial strains viz., Listeria monocytogenes (MTCC657), Staphylococcus aureus (MTCC7443), Pseudomonas aeruginosa (MTCC424), Klebsiella pneumoniae (MTCC432) and Salmonella typhi (MTCC733). However, Escherichia coli (MTCC118), was found resistant to all the fractions. In FT-IR spectroscopy, functional groups like alcohol, amide, alkene, alkyl halide, polysulfide and phosphate ions were identified. The GC-MS analysis of EW1 fraction exhibited the presence of 12 compounds, of which 1-heneicosanol was found as the major compound. These compounds might be responsible synergistically or individually for antibacterial activity of cow urine. Nine elements namely sodium (Na), calcium (Ca), chromium (Cr), iron (Fe), magnesium (Mg), aluminium (Al), potassium (K) and zinc (Zn), Gold (Au) were measured by ICP-MS analysis.