First case of canine paraprostatic infection caused by a high-risk ST147 Klebsiella pneumoniae harboring multiple extended-spectrum-β-lactamase genes.

Multidrug-resistant (MDR) Klebsiella pneumoniae, particularly high-risk clones such as sequence type (ST) 147, have been increasingly reported worldwide. However, data on their occurrence in reproductive disorders of companion animals remain limited. This study describes, for the first time, an extended-spectrum β-lactamase (ESBL)-producing K. pneumoniae ST147 isolated from a canine paraprostatic abscess. The isolate (UNISA-DOG-2025) was characterized by antimicrobial susceptibility testing and whole-genome sequencing. Phenotypic analysis revealed an MDR profile, supported by the detection of multiple resistance genes, including β-lactamases (blaCTX-M-8, blaCTX-M-15, blaOXA-1, blaOXA-9), as well as determinants conferring resistance to aminoglycosides, quinolones, sulfonamides, tetracyclines, and trimethoprim. Several of these genes were located on an in silico predicted conjugative IncM1 plasmid, suggesting potential for horizontal dissemination. Phylogenomic analysis demonstrated close relatedness to globally distributed ST147 strains, predominantly associated with human infections. To our knowledge, this is the first report of K. pneumoniae ST147 causing a canine paraprostatic abscess. These findings highlight the clinical relevance of MDR Enterobacterales in canine reproductive disorders and reinforce the need for antimicrobial stewardship and genomic surveillance in veterinary medicine.