Open veterinary journal · 2026
New gel formulation for treating wounds infected with common bacteria isolated from feline wounds in rats.
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Saleh Mena Mobasher, Ibrahim Orooba Muhammad Saeed
Аннотация
BACKGROUND: The rising need for adjunctive plant-based wound therapeutics with anti-inflammatory, antibacterial, and prohealing properties stems from the growing concern of antibiotic-resistant wound infections in companion animals.
AIM: To determine the main bacterial agents in feline wound infections and to test a new polyherbal gel made from Punica granatum peel, Rosmarinus officinalis leaves, and Cucurbita moschata fibers on Staphylococcus aureus-infected rat wounds model.
METHODS: VITEK-2 was used to confirm the results of culturing and blood, MacConkey, and Mannitol salt agars from veterinary clinics, blood collection camps in Baghdad. The plant materials were Soxhlet-extracted, and polyphenols were analyzed by high-performance liquid chromatography. There were five groups of rats, which included the non-infected control, the infected untreated control, the infected and 6% gel, the infected and 12% gel, and the infected and 2% fucidin. Carbopol gel with 6% (GF6%) and 12% (GF12%) total extracts was macerated and administered once a day for 10 days to excisional wounds infected with S. aureus (1.5 × 108 CFU/ml). The outcome measures were the percentage of wound contraction, absolute values of hydroxyproline (Hyp), ELISA values of vascular endothelial growth factor (VEGF), tumor necrosis factor-α (TNF-α), and histopathological examination.
RESULTS: Staphylococcus aureus was the most prominent isolate (52.8% overall prevalence, most in accidental and postsurgical wounds). The extracts were rich in polyphenols, and GF12% (among other extracts) was able to achieve complete wound closure by day 10 (100%), whereas fucidin 2% and infected controls were 34% and GF6% 97%, respectively. GF12% greatly lowered VEGF and TNF-a levels relative to controls and fucidin while markedly elevating Hyp, suggesting increased collagen deposition. Histology after treatment with GF12% demonstrated orderly reepithelialization and collagen deposition akin to normal skin, whereas immature granulation and persistent inflammation were observed in fucidin-treated wounds.
CONCLUSION: The polyherbal gel, especially at 12% concentration, had the most healing effect on wounds infected with S. aureus due to its powerful antibacterial, anti-inflammatory, collagen-restoring, and anti-angiogenic properties, presenting significant potential for topical use in veterinary wound care.
AIM: To determine the main bacterial agents in feline wound infections and to test a new polyherbal gel made from Punica granatum peel, Rosmarinus officinalis leaves, and Cucurbita moschata fibers on Staphylococcus aureus-infected rat wounds model.
METHODS: VITEK-2 was used to confirm the results of culturing and blood, MacConkey, and Mannitol salt agars from veterinary clinics, blood collection camps in Baghdad. The plant materials were Soxhlet-extracted, and polyphenols were analyzed by high-performance liquid chromatography. There were five groups of rats, which included the non-infected control, the infected untreated control, the infected and 6% gel, the infected and 12% gel, and the infected and 2% fucidin. Carbopol gel with 6% (GF6%) and 12% (GF12%) total extracts was macerated and administered once a day for 10 days to excisional wounds infected with S. aureus (1.5 × 108 CFU/ml). The outcome measures were the percentage of wound contraction, absolute values of hydroxyproline (Hyp), ELISA values of vascular endothelial growth factor (VEGF), tumor necrosis factor-α (TNF-α), and histopathological examination.
RESULTS: Staphylococcus aureus was the most prominent isolate (52.8% overall prevalence, most in accidental and postsurgical wounds). The extracts were rich in polyphenols, and GF12% (among other extracts) was able to achieve complete wound closure by day 10 (100%), whereas fucidin 2% and infected controls were 34% and GF6% 97%, respectively. GF12% greatly lowered VEGF and TNF-a levels relative to controls and fucidin while markedly elevating Hyp, suggesting increased collagen deposition. Histology after treatment with GF12% demonstrated orderly reepithelialization and collagen deposition akin to normal skin, whereas immature granulation and persistent inflammation were observed in fucidin-treated wounds.
CONCLUSION: The polyherbal gel, especially at 12% concentration, had the most healing effect on wounds infected with S. aureus due to its powerful antibacterial, anti-inflammatory, collagen-restoring, and anti-angiogenic properties, presenting significant potential for topical use in veterinary wound care.